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Anthocyanin Partition in Aqueous Two-Phase Systems Based on Isopropanol and Sodium/Ammonium Sulfate
Chemical Engineering & Technology ( IF 1.8 ) Pub Date : 2022-07-13 , DOI: 10.1002/ceat.202100524 Ana Clara Rocha Caldeira 1 , Attilio Converti 2 , Moysés Naves de Moraes 3 , Janaína Teles de Faria 1
Chemical Engineering & Technology ( IF 1.8 ) Pub Date : 2022-07-13 , DOI: 10.1002/ceat.202100524 Ana Clara Rocha Caldeira 1 , Attilio Converti 2 , Moysés Naves de Moraes 3 , Janaína Teles de Faria 1
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Partitioning of anthocyanins contained in the crude extract of Syzygium cumini fruit was investigated in aqueous two-phase systems (ATPS) made up of isopropanol and Na2SO4/(NH4)2SO4. Na2SO4 induced ATPS formation more effectively than (NH4)2SO4. The increase in temperature enhanced phase separation in the (NH4)2SO4 ATPS, while the opposite occurred in the Na2SO4 ATPS. The higher the overall mixture concentration or temperature, the higher the values of tie-line length and slope. In all systems and conditions, anthocyanins preferentially partitioned to the top phase. The partition coefficient and theoretical recovery yield in the top phase varied in the ranges 1.14–1.77 and 53.31–63.87 % in the (NH4)2SO4 ATPS, and in the ranges 1.94–21.50 and 65.90–95.55 % in the Na2SO4 ATPS.
中文翻译：
基于异丙醇和硫酸钠/硫酸铵的水相两相体系中的花青素分配
在由异丙醇和 Na 2 SO 4 /(NH 4 ) 2 SO 4组成的水性两相系统 (ATPS) 中研究了蒲桃果实粗提物中所含花青素的分配。Na 2 SO 4比(NH 4 ) 2 SO 4更有效地诱导ATPS形成。(NH 4 ) 2 SO 4 ATPS中温度的升高增强了相分离，而在 Na 2 SO 4中则相反。ATP。总混合物浓度或温度越高，连接线长度和斜率的值就越高。在所有系统和条件下，花青素优先分配到顶层。(NH 4 ) 2 SO 4 ATPS顶相的分配系数和理论回收率在 1.14-1.77 和 53.31-63.87 %范围内变化，而 Na 2则在 1.94-21.50 和 65.90-95.55 % 范围内变化SO 4 ATPS。
更新日期：2022-07-13
中文翻译：
基于异丙醇和硫酸钠/硫酸铵的水相两相体系中的花青素分配
在由异丙醇和 Na 2 SO 4 /(NH 4 ) 2 SO 4组成的水性两相系统 (ATPS) 中研究了蒲桃果实粗提物中所含花青素的分配。Na 2 SO 4比(NH 4 ) 2 SO 4更有效地诱导ATPS形成。(NH 4 ) 2 SO 4 ATPS中温度的升高增强了相分离，而在 Na 2 SO 4中则相反。ATP。总混合物浓度或温度越高，连接线长度和斜率的值就越高。在所有系统和条件下，花青素优先分配到顶层。(NH 4 ) 2 SO 4 ATPS顶相的分配系数和理论回收率在 1.14-1.77 和 53.31-63.87 %范围内变化，而 Na 2则在 1.94-21.50 和 65.90-95.55 % 范围内变化SO 4 ATPS。
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