当前位置: X-MOL 学术Biotechnol. Bioeng. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Physiological suitability of sulfate-reducing granules for the development of bioconcrete
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2022-07-16 , DOI: 10.1002/bit.28184
Kirthi Chetty 1 , Timothy McCarthy 1 , Faisal Hai 1 , Shuxin Zhang 1 , Yarong Song 2 , Guangming Jiang 1
Affiliation  

Regular monitoring and timely repair of concrete cracks are required to minimize further deterioration. Self-healing of cracks has been proposed as an alternative to the crack maintenance procedures. One of the proposed techniques is to use axenic cultures to exploit microbial-induced calcite precipitation (MICP). However, such healing agents are not cost-effective for in situ use. As the market for bio-based self-healing concrete necessitates a low-cost bio-agent, nonaxenic sulfate reducing bacterial (SRB) granules were investigated in this study through cultivation in an upflow anaerobic sludge blanket reactor. The compact granules can protect the bacteria from adverse conditions without encapsulation. This study investigated the microbial activities of SRB granules at different temperatures, pH, and chemical oxygen demand concentrations which the microbes would experience during the concrete casting and curing process. The attenuation and recovery of microbial activities were measured before and after the exposure. Moreover, the MICP yield was also tested for a possible use in self-healing bioconcrete. The results consistently showed that SRB granules were able to survive starvation, high temperature (50–60°C), and high pH (12), together with scanning electron microscope/energy dispersive spectrometry/X-ray diffraction analysis evidence. Microbial staining analysis demonstrated the formation of spores in the granules during their exposure to harsh conditions. SRB granule was thus demonstrated to be a viable self-healing nonaxenic agent for low-cost bioconcrete.

中文翻译:

硫酸盐还原颗粒对生物混凝土开发的生理适用性

需要定期监测和及时修复混凝土裂缝,以尽量减少进一步恶化。已经提出裂缝的自我修复作为裂缝维护程序的替代方案。提议的技术之一是使用无菌培养来利用微生物诱导的方解石沉淀 (MICP)。然而,这样的愈合剂对于原位使用来说不具有成本效益。由于生物基自修复混凝土市场需要低成本的生物制剂,本研究通过在上流式厌氧污泥床反应器中培养来研究非无菌硫酸盐还原细菌 (SRB) 颗粒。致密的颗粒可以在不包封的情况下保护细菌免受不利条件的影响。本研究调查了 SRB 颗粒在不同温度、pH、以及微生物在混凝土浇注和养护过程中所经历的化学需氧量浓度。在暴露前后测量微生物活性的衰减和恢复。此外,还测试了 MICP 产量是否可能用于自修复生物混凝土。结果一致表明,SRB 颗粒能够在饥饿、高温(50-60°C)和高 pH 值(12)中存活,并结合扫描电子显微镜/能量色散光谱法/X 射线衍射分析证据。微生物染色分析表明,在暴露于恶劣条件下时,颗粒中会形成孢子。因此,SRB 颗粒被证明是一种可行的自愈非无菌剂,可用于低成本的生物混凝土。在暴露前后测量微生物活性的衰减和恢复。此外,还测试了 MICP 产量是否可能用于自修复生物混凝土。结果一致表明,SRB 颗粒能够在饥饿、高温(50-60°C)和高 pH 值(12)中存活,并结合扫描电子显微镜/能量色散光谱法/X 射线衍射分析证据。微生物染色分析表明,在暴露于恶劣条件下时,颗粒中会形成孢子。因此,SRB 颗粒被证明是一种可行的自愈非无菌剂,可用于低成本的生物混凝土。在暴露前后测量微生物活性的衰减和恢复。此外,还测试了 MICP 产量是否可能用于自修复生物混凝土。结果一致表明,SRB 颗粒能够在饥饿、高温(50-60°C)和高 pH 值(12)中存活,并结合扫描电子显微镜/能量色散光谱法/X 射线衍射分析证据。微生物染色分析表明,在暴露于恶劣条件下时,颗粒中会形成孢子。因此,SRB 颗粒被证明是一种可行的自愈非无菌剂,可用于低成本的生物混凝土。结果一致表明,SRB 颗粒能够在饥饿、高温(50-60°C)和高 pH 值(12)中存活,并结合扫描电子显微镜/能量色散光谱法/X 射线衍射分析证据。微生物染色分析表明,在暴露于恶劣条件下时,颗粒中会形成孢子。因此,SRB 颗粒被证明是一种可行的自愈非无菌剂,可用于低成本的生物混凝土。结果一致表明,SRB 颗粒能够在饥饿、高温(50-60°C)和高 pH 值(12)中存活,并结合扫描电子显微镜/能量色散光谱法/X 射线衍射分析证据。微生物染色分析表明,在暴露于恶劣条件下时,颗粒中会形成孢子。因此,SRB 颗粒被证明是一种可行的自愈非无菌剂,可用于低成本的生物混凝土。
更新日期:2022-07-16
down
wechat
bug