Recent advances in yeast genome evolution with stress tolerance for green biological manufacturing,Biotechnology and Bioengineering

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Recent advances in yeast genome evolution with stress tolerance for green biological manufacturing
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2022-07-15 , DOI: 10.1002/bit.28183
Ke Xu _{1,

2} , Yun-Feng Zhang ₁ , Dong-Yu Guo ₁ , Lei Qin ₂ , Munaza Ashraf ₃ , Nadeem Ahmad ₄

Affiliation

Green biological manufacturing is a revolutionary industrial model utilizing yeast as a significant microbial cell factory to produce biofuels and other biochemicals. However, biotransformation efficiency is often limited owing to several stress factors resulting from environmental changes or metabolic imbalance, leading to the slow growth of cells, compromised yield, and enhanced energy consumption. These factors make biological manufacturing competitively less economical. In this regard, minimizing the stress impact on microbial cell factories and strong robust performance have been an interesting area of interest in the last few decades. In this review, we focused on revealing the stress factors and their associated mechanisms for yeast in biological manufacturing. To improve yeast tolerance, rational and irrational strategies were introduced, and the molecular basis of genome evolution in yeast was also summarized. Furthermore, strategies of genome-directed evolution such as homology directed repair and nonhomologous end-joining, and the synthetic chromosome recombination and modification by LoxP-mediated evolution and their association with stress tolerance was highlighted. We hope that genome evolution provides new insights for solving the limitations of the natural phenotypes of microorganisms in industrial fermentation for the production of valuable compounds.

中文翻译：

绿色生物制造抗逆性酵母基因组进化的最新进展

绿色生物制造是一种革命性的工业模式，利用酵母作为重要的微生物细胞工厂生产生物燃料和其他生物化学品。然而，由于环境变化或代谢失衡导致的多种应激因素，生物转化效率往往受到限制，导致细胞生长缓慢、产量下降和能量消耗增加。这些因素使生物制造在竞争中较不经济。在这方面，在过去几十年中，最大限度地减少对微生物细胞工厂的压力影响和强大的稳健性能一直是一个有趣的领域。在这篇综述中，我们重点揭示了酵母在生物制造中的压力因素及其相关机制。为了提高酵母耐受性，引入了合理和非合理的策略，并对酵母基因组进化的分子基础进行了总结。此外，强调了基因组定向进化的策略，例如同源定向修复和非同源末端连接，以及 LoxP 介导的进化合成染色体重组和修饰及其与胁迫耐受性的关联。我们希望基因组进化为解决工业发酵中微生物自然表型的局限性提供新的见解，以生产有价值的化合物。并强调了通过 LoxP 介导的进化进行的合成染色体重组和修饰及其与胁迫耐受性的关联。我们希望基因组进化为解决工业发酵中微生物自然表型的局限性提供新的见解，以生产有价值的化合物。并强调了通过 LoxP 介导的进化进行的合成染色体重组和修饰及其与胁迫耐受性的关联。我们希望基因组进化为解决工业发酵中微生物自然表型的局限性提供新的见解，以生产有价值的化合物。

更新日期：2022-07-15

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