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Observations on the cytomorphology and ultrastructure of the peripheral blood cells of native cattle (Zobawng) of Mizoram, India
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2022-07-13 , DOI: 10.1002/jemt.24197 Rupan Sarkar 1 , Arup Kalita 1 , Om Prakash Choudhary 1 , Pranab Chandra Kalita 1 , Probal Jyoti Doley 1 , Swarup Debroy 1
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2022-07-13 , DOI: 10.1002/jemt.24197 Rupan Sarkar 1 , Arup Kalita 1 , Om Prakash Choudhary 1 , Pranab Chandra Kalita 1 , Probal Jyoti Doley 1 , Swarup Debroy 1
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This study aims to characterize the cytomorphology and ultrastructure of blood cells of native cattle of Mizoram. Twelve numbers of blood samples (10 ml) were collected from the Zobawng cattle, irrespective of sex. Blood smears were prepared and stained with different stains for cytomorphological study. The standard protocol has been followed for preparing blood samples for electron microscopy. Under a light microscope, erythrocytes of cattle were non-nucleated and round. The neutrophils were round, and the cytoplasm contained cytoplasmic granules. The eosinophils were rounded in outline with distinct cytoplasmic granules. The presence of basophils was infrequent with distinct blue color cytoplasmic granules. Small, medium, and large types of lymphocytes were recorded. The monocytes were round to oval in outline. Platelets were irregular to round. The reticulocytes were recorded occasionally, like small blue thin rods or granules. The cytoplasm and nucleus of granulocytes fluoresced greenish-yellow and red, respectively, with supravital stain. Under scanning electron microscopy (SEM), the erythrocytes appeared as biconcave discs. Different leukocytes were observed with their finger-like, plate-like, and narrow cell processes on their surface. Platelets were irregular structures. In transmission electron microscopy (TEM), the erythrocytes appeared anucleated biconcave elongated, neutrophils were roughly rounded with small cytoplasmic processes, and eosinophils were roughly circular with small cytoplasmic processes, the basophils were roughly circular with oval to elongated cytoplasmic granules, lymphocytes were roughly circular with centrally placed well-marked oval indented nucleus and some cytoplasmic processes, monocytes appeared spherical with long thick cytoplasmic processes and the non-nucleated platelets appeared roughly round to elongated.
中文翻译：
印度米佐拉姆邦本地牛（Zobawng）外周血细胞的细胞形态学和超微结构观察
本研究旨在表征米佐拉姆邦本地牛血细胞的细胞形态学和超微结构。从 Zobowng 牛采集了 12 份血样（10 毫升），不分性别。制备血涂片并用不同的染料染色用于细胞形态学研究。为电子显微镜准备血液样本已遵循标准协议。光镜下，牛红细胞无核，呈圆形。中性粒细胞呈圆形，胞浆内含胞浆颗粒。嗜酸性粒细胞轮廓呈圆形，具有明显的细胞质颗粒。嗜碱性粒细胞的存在是罕见的，具有明显的蓝色细胞质颗粒。记录小型、中型和大型淋巴细胞。单核细胞的轮廓为圆形至椭圆形。血小板不规则至圆形。偶尔记录网织红细胞，像小的蓝色细棒或颗粒。粒细胞的细胞质和细胞核分别发出黄绿色和红色荧光，有超生体染色。在扫描电子显微镜（SEM）下，红细胞表现为双凹圆盘。观察到不同的白细胞在其表面具有指状、板状和狭窄的细胞突起。血小板为不规则结构。透射电镜（TEM）下，红细胞呈无核双凹拉长，中性粒细胞大致呈圆形，有小胞浆突起，嗜酸性粒细胞大致呈圆形，有小胞浆突起，嗜碱性粒细胞大致呈圆形，呈椭圆形至细长的胞质颗粒，
更新日期：2022-07-13
中文翻译：
印度米佐拉姆邦本地牛（Zobawng）外周血细胞的细胞形态学和超微结构观察
本研究旨在表征米佐拉姆邦本地牛血细胞的细胞形态学和超微结构。从 Zobowng 牛采集了 12 份血样（10 毫升），不分性别。制备血涂片并用不同的染料染色用于细胞形态学研究。为电子显微镜准备血液样本已遵循标准协议。光镜下，牛红细胞无核，呈圆形。中性粒细胞呈圆形，胞浆内含胞浆颗粒。嗜酸性粒细胞轮廓呈圆形，具有明显的细胞质颗粒。嗜碱性粒细胞的存在是罕见的，具有明显的蓝色细胞质颗粒。记录小型、中型和大型淋巴细胞。单核细胞的轮廓为圆形至椭圆形。血小板不规则至圆形。偶尔记录网织红细胞，像小的蓝色细棒或颗粒。粒细胞的细胞质和细胞核分别发出黄绿色和红色荧光，有超生体染色。在扫描电子显微镜（SEM）下，红细胞表现为双凹圆盘。观察到不同的白细胞在其表面具有指状、板状和狭窄的细胞突起。血小板为不规则结构。透射电镜（TEM）下，红细胞呈无核双凹拉长，中性粒细胞大致呈圆形，有小胞浆突起，嗜酸性粒细胞大致呈圆形，有小胞浆突起，嗜碱性粒细胞大致呈圆形，呈椭圆形至细长的胞质颗粒，
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