Perfluorinated compounds (PFCs) are a group of widely used synthetic chemicals. Owing to their unique chemical properties, PFCs can accumulate in the environment and living organisms. In vitro and in vivo studies have demonstrated the adverse effects of exposure to PFCs, resulting in increased concern. Therefore, a fast, reliable analytical method is crucial for human biomonitoring and health risk assessment. This study used two isotope internal standards to identify and quantify 24 PFCs in umbilical cord serum samples, based on classical liquid–liquid extraction (LLE) with liquid chromatography tandem mass spectrometry (LC-MS/MS). According to our review of the literature, this study is the first to determine the TFHSA, S4hPDS, S4hPOS, S4hPHS, SPHeS, SPNoS, and SPPeS by using this developed method. The average spiked recoveries of 24 PFCs were acceptable, ranging from approximately 64.0% to 124%; RSDs ranged from 0.74% to 11.2%; LOD and LOQ ranged from 0.013 to 0.248 μg/L and from 0.030 to 0.747 μg/L, respectively. This method was applied to measure the PFCs in umbilical cord serum samples; 24 PFCs were detected in the investigated samples, which are comparable to those reported in the literature. TFHSA, S4hPDS, S4hPOS, S4hPHS, SPHeS, SPNoS, and SPPeS were also detected in the samples, which should be investigated in further research. The sensitivity, accuracy, and precision of the developed method are sufficient for its application in large-scale biomonitoring studies.

全氟化合物 (PFC) 是一组广泛使用的合成化学品。由于其独特的化学性质，PFCs 可以在环境和生物体内积累。体外和体内_研究表明接触 PFC 会产生不利影响，从而引起更多关注。因此，快速、可靠的分析方法对于人体生物监测和健康风险评估至关重要。本研究基于经典液液萃取 (LLE) 和液相色谱串联质谱 (LC-MS/MS)，使用两种同位素内标来鉴定和定量脐带血清样品中的 24 种 PFC。根据我们对文献的回顾，本研究首次使用这种开发的方法确定了 TFHSA、S4hPDS、S4hPOS、S4hPHS、SPHeS、SPNoS 和 SPPeS。24 种 PFC 的平均加标回收率是可以接受的，范围从大约 64.0% 到 124%；RSD 范围为 0.74% 至 11.2%；LOD 和 LOQ 的范围分别为 0.013 至 0.248 μg/L 和 0.030 至 0.747 μg/L。该方法应用于脐带血清样品中PFCs的测定；在调查的样品中检测到 24 种 PFC，与文献报道的结果相当。样品中还检测到 TFHSA、S4hPDS、S4hPOS、S4hPHS、SPHeS、SPNoS 和 SPPeS，应在进一步研究中进行调查。所开发方法的灵敏度、准确性和精密度足以使其在大规模生物监测研究中的应用。