Abstract

Astaxanthin is a natural carotenoid with strong antioxidant activity. In this paper, the effects of carbon source, corn steep liquor, distiller grains, and initial pH on the growth and astaxanthin production of Phaffia rhodozyma D3 were evaluated. The optimal medium composition was 32 g/L glucose, 12 g/L corn steep liquor as nitrogen source, and the initial pH was 6.7. Phaffia rhodozyma D3 was cultured in a shake flask under these optimized conditions, the biomass was 6.47 g/L, the astaxanthin/OD₄₇₅ was 15.16, and the astaxanthin content was 1.41 mg/g. The astaxanthin content was further increased to 4.70 mg/g by the combination of TiO₂ stimulation and the expanding cultivation of P. rhodozyma D3 in a 5 L fermenter, which was 2.81 times that of the control group. Expanding fermentation implies the possibility of large-scale production in the astaxanthin industry. Corn steep liquor was used as an alternative nitrogen source to culture P. rhodozyma D3, which could both reduce the production cost of astaxanthin and increased the by-products utilization rate.

摘要

虾青素是一种天然的类胡萝卜素，具有很强的抗氧化活性。本文研究了碳源、玉米浆、酒糟和初始pH值对红发夫酵母D3生长和虾青素产量的影响。最佳培养基组成为32 g/L葡萄糖，12 g/L玉米浆为氮源，初始pH为6.7。在此优化条件下，红发夫酵母D3在摇瓶中培养，生物量为6.47 g/L，虾青素/OD ₄₇₅为15.16，虾青素含量为1.41 mg/g。通过TiO _2刺激和P. rhodozyma的扩大培养相结合，虾青素含量进一步提高到4.70 mg/gD3在5L发酵罐中，是对照组的2.81倍。扩大发酵意味着虾青素产业规模化生产的可能性。以玉米浆作为替代氮源培养P. rhodozyma D3，既可以降低虾青素的生产成本，又可以提高副产物的利用率。