Rapid nucleic acid extraction from skin biopsies using a point-of-care device,Lab on a Chip

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Rapid nucleic acid extraction from skin biopsies using a point-of-care device
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-07-13 , DOI: 10.1039/d2lc00457g
Duncan McCloskey ₁ , David Erickson _{2,

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Affiliation

Sample processing is often the rate-limiting step for point-of-care nucleic acid testing, especially for large, robust tissues such as skin biopsies, which can be used to diagnose a variety of dermatological diseases. Extraction of nucleic acids from these samples often relies on lengthy enzymatic digestions, increasing the time to result and reducing the potential impact of rapid molecular diagnostic approaches. To address this, we have developed BLENDER, a device for rapid nucleic acid extraction from tissue biopsies that combines bead-beating homogenization with simultaneous sample heating for enzymatic lysis. Our device can produce a complete DNA yield from a 3 mm cylindrical skin biopsy with only a 15 minute extraction compared to 4 hours when using a commercially available extraction protocol. Decreasing sample-processing time for tissue biopsies could reduce time-to-result for downstream analysis, enabling faster point-of-care diagnosis of solid cancers in limited resource settings.

中文翻译：

使用护理点设备从皮肤活检中快速提取核酸

样品处理通常是即时核酸检测的限速步骤，特别是对于皮肤活检等大型、坚固的组织，其可用于诊断各种皮肤病。从这些样品中提取核酸通常依赖于长时间的酶消化，这增加了获得结果的时间并减少了快速分子诊断方法的潜在影响。为了解决这个问题，我们开发了 BLENDER，这是一种从组织活检中快速提取核酸的设备，它将珠打匀化与同时样品加热进行酶裂解相结合。我们的设备只需 15 分钟即可从 3 毫米圆柱形皮肤活检中提取完整的 DNA，而使用市售提取方案则需要 4 小时。减少组织活检的样本处理时间可以缩短下游分析的结果时间，从而能够在有限的资源环境中更快地进行实体癌的护理点诊断。

更新日期：2022-07-13

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