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Three-dimensional heating and patterning dynamics of particles in microscale acoustic tweezers
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-07-12 , DOI: 10.1039/d2lc00200k Robert Weser 1 , Zhichao Deng 2 , Vijay V Kondalkar 1 , Alexandre N Darinskii 3 , Christian Cierpka 2 , Hagen Schmidt 1 , Jörg König 2
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-07-12 , DOI: 10.1039/d2lc00200k Robert Weser 1 , Zhichao Deng 2 , Vijay V Kondalkar 1 , Alexandre N Darinskii 3 , Christian Cierpka 2 , Hagen Schmidt 1 , Jörg König 2
Affiliation
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Acoustic tweezers facilitate a noninvasive, contactless, and label-free method for the precise manipulation of micro objects, including biological cells. Although cells are exposed to mechanical and thermal stress, acoustic tweezers are usually considered as biocompatible. Here, we present a holistic experimental approach to reveal the correlation between acoustic fields, acoustophoretic motion and heating effects of particles induced by an acoustic tweezer setup. The system is based on surface acoustic waves and was characterized by applying laser Doppler vibrometry, astigmatism particle tracking velocimetry and luminescence lifetime imaging. In situ measurements with high spatial and temporal resolution reveal a three-dimensional particle patterning coinciding with the experimentally assisted numerical result of the acoustic radiation force distribution. In addition, a considerable and rapid heating up to 55 °C depending on specific parameters was observed. Although these temperatures may be harmful to living cells, counter-measures can be found as the time scales of patterning and heating are shown to be different.
中文翻译：
微尺度声镊中粒子的三维加热和图案化动力学
声学镊子促进了一种无创、非接触和无标记的方法，用于精确操纵包括生物细胞在内的微型物体。尽管细胞会受到机械和热应力，但声学镊子通常被认为具有生物相容性。在这里，我们提出了一种整体实验方法来揭示声场、声泳运动和声学镊子装置引起的粒子加热效应之间的相关性。该系统以表面声波为基础，采用激光多普勒测振仪、散光粒子跟踪测速仪和发光寿命成像进行表征。原位具有高空间和时间分辨率的测量揭示了与声辐射力分布的实验辅助数值结果一致的三维粒子图案。此外，根据特定参数，观察到可观且快速加热至 55 °C。虽然这些温度可能对活细胞有害，但可以找到对策，因为图案化和加热的时间尺度不同。
更新日期：2022-07-12
中文翻译：
微尺度声镊中粒子的三维加热和图案化动力学
声学镊子促进了一种无创、非接触和无标记的方法，用于精确操纵包括生物细胞在内的微型物体。尽管细胞会受到机械和热应力，但声学镊子通常被认为具有生物相容性。在这里，我们提出了一种整体实验方法来揭示声场、声泳运动和声学镊子装置引起的粒子加热效应之间的相关性。该系统以表面声波为基础，采用激光多普勒测振仪、散光粒子跟踪测速仪和发光寿命成像进行表征。原位具有高空间和时间分辨率的测量揭示了与声辐射力分布的实验辅助数值结果一致的三维粒子图案。此外，根据特定参数，观察到可观且快速加热至 55 °C。虽然这些温度可能对活细胞有害，但可以找到对策，因为图案化和加热的时间尺度不同。
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