Preparing phosphorylated peptides with multiple adjacent phosphorylations is synthetically difficult, leads to β-elimination, results in low yields, and is extremely slow. We combined synthetic chemical methodologies with computational studies and engineering approaches to develop a strategy that takes advantage of fast stirring, high temperature, and a very low concentration of 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) to produce multiphosphorylated peptides at an extremely rapid time and high purity.

中文翻译：

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加速多磷酸化肽合成

制备具有多个相邻磷酸化的磷酸化肽在合成上是困难的，会导致 β-消除，导致产量低，而且速度极慢。我们将合成化学方法与计算研究和工程方法相结合，开发了一种利用快速搅拌、高温和极低浓度的 1,8-二氮杂双环[5.4.0]undec-7-ene (DBU) 的策略以极快的速度和高纯度生产多磷酸化肽。