Optical coherence tomography (OCT) has seen widespread success as an in vivo clinical diagnostic 3D imaging modality, impacting areas including ophthalmology, cardiology, and gastroenterology. Despite its many advantages, such as high sensitivity, speed, and depth penetration, OCT suffers from several shortcomings that ultimately limit its utility as a 3D microscopy tool, such as its pervasive coherent speckle noise and poor lateral resolution required to maintain millimeter-scale imaging depths. Here, we present 3D optical coherence refraction tomography (OCRT), a computational extension of OCT that synthesizes an incoherent contrast mechanism by combining multiple OCT volumes, acquired across two rotation axes, to form a resolution-enhanced, speckle-reduced, refraction-corrected 3D reconstruction. Our label-free computational 3D microscope features a novel optical design incorporating a parabolic mirror to enable the capture of 5D plenoptic datasets, consisting of millimetric 3D fields of view over up to ±75∘${\pm}{75^ \circ}$ without moving the sample. We demonstrate that 3D OCRT reveals 3D features unobserved by conventional OCT in fruit fly, zebrafish, and mouse samples.

中文翻译：

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具有光学相干折射断层扫描的计算 3D 显微镜

光学相干断层扫描 (OCT) 作为一种体内临床诊断 3D 成像方式已取得广泛成功，影响了眼科、心脏病学和胃肠病学等领域。尽管 OCT 具有许多优点，例如高灵敏度、速度快和深度穿透，但它也存在一些缺点，这些缺点最终限制了其作为 3D 显微镜工具的实用性，例如其普遍存在的相干散斑噪声以及维持毫米级成像所需的横向分辨率较差深处。在这里，我们提出了 3D 光学相干折射断层扫描 (OCRT)，这是 OCT 的计算扩展，通过组合跨两个旋转轴获取的多个 OCT 体积来合成不相干对比机制，形成分辨率增强、散斑减少、折射校正的图像3D 重建。我们的无标签计算 3D 显微镜采用新颖的光学设计，结合抛物面镜，能够捕获 5D 全光数据集，其中包含高达 ± 75 ∘ 的毫米级 3D 视场，而无需移动样品${\pm}{75^ \circ}$。我们证明，3D OCRT 揭示了果蝇、斑马鱼和小鼠样本中传统 OCT 未观察到的 3D 特征。