Prototype Digital Lateral Flow Sensor Using Impact Electrochemistry in a Competitive Binding Assay,ACS Sensors

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Prototype Digital Lateral Flow Sensor Using Impact Electrochemistry in a Competitive Binding Assay
ACS Sensors ( IF 8.2 ) Pub Date : 2022-07-08 , DOI: 10.1021/acssensors.2c00728
Lennart J K Weiß ₁ , Philipp Rinklin ₁ , Bhawana Thakur ₁ , Emir Music ₁ , Heike Url ₁ , Inola Kopic ₁ , Darius Hoven ₁ , Marko Banzet ₂ , Tassilo von Trotha ₁ , Dirk Mayer ₂ , Bernhard Wolfrum ₁

Affiliation

This work demonstrates a lateral flow assay concept on the basis of stochastic-impact electrochemistry. To this end, we first elucidate requirements to employ silver nanoparticles as redox-active labels. Then, we present a prototype that utilizes nanoimpacts from biotinylated silver nanoparticles as readouts to detect free biotin in solution based on competitive binding. The detection is performed in a membrane-based microfluidic system, where free biotin and biotinylated particles compete for streptavidin immobilized on embedded latex beads. Excess nanoparticles are then registered downstream at an array of detection electrodes. In this way, we establish a proof of concept that serves as a blueprint for future “digital” lateral flow sensors.

中文翻译：

在竞争性结合分析中使用冲击电化学的原型数字横向流量传感器

这项工作展示了基于随机冲击电化学的横向流动分析概念。为此，我们首先阐明了使用银纳米粒子作为氧化还原活性标记的要求。然后，我们提出了一个原型，该原型利用生物素化银纳米粒子的纳米影响作为读数，基于竞争结合检测溶液中的游离生物素。检测是在基于膜的微流体系统中进行的，其中游离的生物素和生物素化颗粒竞争固定在嵌入乳胶珠上的链霉亲和素。然后将多余的纳米颗粒记录在下游的检测电极阵列上。通过这种方式，我们建立了一个概念验证，作为未来“数字”横向流量传感器的蓝图。

更新日期：2022-07-08

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