Chiroptical detection of self-aggregating fluorescent rhodamine conjugates: Mistakes and prospects,Journal of Peptide Science

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Chiroptical detection of self-aggregating fluorescent rhodamine conjugates: Mistakes and prospects
Journal of Peptide Science ( IF 1.8 ) Pub Date : 2022-07-07 , DOI: 10.1002/psc.3442
Ferenc Zsila ₁

Affiliation

Fluorescent conjugation can be considered as the chromophoric derivatization of the target, and as such, it may provide additional structure-related information available by using circular dichroism (CD) spectroscopy. In this essay, peculiar CD spectroscopic data reported earlier for thyroid hormone–rhodamine conjugates have been re-evaluated. Contrary to the original proposal on the intramolecular folding of the labelled hormone, the bisignate motif observed in the CD spectrum is a clear evidence of dye–dye intermolecular chiral exciton coupling, indicating supramolecular self-association of the conjugates. This anomalous solution behaviour undermines the credibility of experimental results reported with such conjugates still being used in the laboratory practice. The extension of routine far-ultraviolet (UV) CD spectroscopic scans of chiral fluorophore conjugates into the near-UV and visible spectral region is strongly recommended.

中文翻译：

自聚集荧光罗丹明缀合物的手性检测：错误和前景

荧光共轭可以被认为是目标的发色衍生化，因此，它可以通过使用圆二色 (CD) 光谱提供额外的结构相关信息。在这篇文章中，之前报道的甲状腺激素-罗丹明结合物的特殊 CD 光谱数据已被重新评估。与最初关于标记激素分子内折叠的提议相反，在 CD 光谱中观察到的双符号基序是染料-染料分子间手性激子偶联的明确证据，表明缀合物的超分子自缔合。这种异常的溶液行为破坏了报告的实验结果的可信度，而这种共轭物仍在实验室实践中使用。

更新日期：2022-07-07

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