Abstract

Ochrobactrum sp. XKL1, previously found to have the ability to efficiently degrade quinoline, was bioaugmented into a lab-scale A/O/O system to treat real coking wastewater. During the bioaugmentation stage, the removal of quinoline and pyridine of the O1 tank could be enhanced by 9.88% and 7.96%, respectively. High-throughput sequencing analysis indicated that the addition of XKL1 could significantly affect the alteration of microbial community structure in the sludge. In addition, the relative abundance of Ochrobactrum has demonstrated a trend of increasing first followed by decreasing with the highest abundance of 7.87% attained on the 94th day. The bioaugmentation effects lasted for about 14 days after the strains was inoculated into the reactor. Although a decrease in the relative abundance of XKL1 was observed for a rather short period of time, the bioaugmented A/O/O system has been proven to be more effective in the removal of organic pollutants than the control. Hence, the results of this study indicated that the bioaugmentation with XKL1 is a feasible operational strategy that would be able to enhance the removal of NHCs in the treatment of coking wastewater with complex composition and high organic concentrations.

摘要

Ochrobactrum sp。XKL1 之前被发现具有有效降解喹啉的能力，它被生物增强到实验室规模的 A/O/O 系统中，以处理真正的焦化废水。在生物强化阶段，O1罐对喹啉和吡啶的去除率分别提高了9.88%和7.96%。高通量测序分析表明，XKL1的加入可以显着影响污泥中微生物群落结构的改变。此外，Ochrobactrum的相对丰度显示出先增加后减少的趋势，在第 94 天达到最高丰度 7.87%。将菌株接种到反应器中后，生物增强作用持续了约 14 天。尽管在相当短的时间内观察到 XKL1 的相对丰度下降，但已证明生物增强 A/O/O 系统在去除有机污染物方面比对照更有效。因此，本研究结果表明，用 XKL1 进行生物强化是一种可行的操作策略，能够在处理具有复杂成分和高有机浓度的焦化废水中增强 NHCs 的去除。