Quantitative Analysis of Exhaled Breath Collected on Filter Substrates via Low-Temperature Plasma Desorption/Ionization Mass Spectrometry,ACS Environmental Au

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Quantitative Analysis of Exhaled Breath Collected on Filter Substrates via Low-Temperature Plasma Desorption/Ionization Mass Spectrometry
ACS Environmental Au ( IF 6.7 ) Pub Date : 2022-07-06 , DOI: 10.1021/jasms.2c00109
Xiaoxia Gong ₁ , Songyue Shi ₁ , Dong Zhang ₁ , Gerardo Gamez ₁

Affiliation

Breath analysis has attracted increasing attention in recent years due to its great potential for disease diagnostics at early stages and for clinical drug monitoring. There are several recent examples of successful development of real-time, in vivo quantitative analysis of exhaled breath metabolites via mass spectrometry. On the other hand, current mass spectrometer accessibility limitations restrict point-of-care applications. Here now, an offline method is developed for quantitative analysis of exhaled breath collected on inexpensive filter substrates for direct desorption and ionization by using low-temperature plasma-mass spectrometry (LTP-MS). In particular, different operating conditions of the ionization source were systematically studied to optimize desorption/ionization by using glycerol, a low volatility compound. Applications with respect to propofol, γ-valprolactone, and nicotine analysis in exhaled breath are demonstrated in this study. The effects of several filter substrate properties, including filter material and pore size, on the analyte signal were characterized. Cellulose filter papers performed best with the present analytes. In addition, filters with smaller pores enabled a more efficient sample collection. Furthermore, sample-collection flow rate was determined to have a very significant effect, with slower flow rates yielding the best results. It was also found that filters loaded with sample can be successfully stored in glass vials with no observable sample loss even after 3 days. Limits of detection under optimized conditions are shown to be competitive or significantly better compared with relevant techniques and with additional benefits of cost-efficiency and sample storage capabilities.

中文翻译：

通过低温等离子解吸/电离质谱法对过滤器基板上收集的呼出气进行定量分析

近年来，呼吸分析因其在疾病早期诊断和临床药物监测方面的巨大潜力而受到越来越多的关注。最近有几个通过质谱法成功开发呼出气代谢物实时体内定量分析的例子。另一方面，当前质谱仪的可访问性限制限制了床旁应用。现在，开发了一种离线方法，用于通过使用低温等离子体质谱 (LTP-MS) 对廉价过滤器基板上收集的呼出气进行定量分析，以进行直接解吸和电离。特别是，系统地研究了电离源的不同操作条件，以通过使用低挥发性化合物甘油来优化解吸/电离。本研究展示了呼气中丙泊酚、γ-丙内酯和尼古丁分析的应用。表征了几种过滤器基材特性（包括过滤器材料和孔径）对分析物信号的影响。纤维素滤纸对本分析物表现最好。此外，具有更小孔的过滤器能够更有效地收集样品。此外，样品收集流速被确定具有非常显着的影响，较慢的流速产生最佳结果。还发现装有样品的过滤器可以成功地储存在玻璃瓶中，即使在 3 天后也没有观察到样品损失。

更新日期：2022-07-06

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