Home-Built Spinning Apparatus for Drying Agarose-Based Imaging Mass Spectrometry Samples,ACS Environmental Au

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Home-Built Spinning Apparatus for Drying Agarose-Based Imaging Mass Spectrometry Samples
ACS Environmental Au ( IF 6.7 ) Pub Date : 2022-05-31 , DOI: 10.1021/jasms.2c00044
Hannah J. Lusk ₁ , Sarah E. Levy ₁ , Tova M. Bergsten ₂ , Joanna E. Burdette ₂ , Laura M. Sanchez ₁

Affiliation

Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a useful technique for mapping the spatial distribution of molecules across biological samples. Sample preparation is crucial for MALDI-IMS; samples must be flat, dry, and cocrystallized with a matrix prior to analysis. Agarose-based samples can be difficult to consistently prepare as they are susceptible to environmental changes, which can lead to inconsistent drying and wrinkling on the sample surface. Small height differences may cause low ionization of target analytes or introduce artifacts in imaging data depending on the instrument used for analysis. To overcome the variations, a home-built robotic spinner was constructed and applied to agarose-based samples. This robotic spinner is inexpensive and easy to assemble, and when it was applied to agarose-based samples, accelerated the drying process and reduced wrinkles, improving the overall quality of the resulting IMS data.

中文翻译：

用于干燥基于琼脂糖的成像质谱样品的自制旋转装置

基质辅助激光解吸/电离 (MALDI) 成像质谱 (IMS) 是一种有用的技术，用于绘制生物样品中分子的空间分布。样品制备对于 MALDI-IMS 至关重要；样品在分析前必须平整、干燥并与基质共结晶。基于琼脂糖的样品可能难以始终如一地制备，因为它们容易受到环境变化的影响，这可能导致样品表面干燥不一致和起皱。取决于用于分析的仪器，小的高度差异可能会导致目标分析物的低电离或在成像数据中引入伪影。为了克服这些变化，构建了一个自制的机器人旋转器并将其应用于基于琼脂糖的样品。这种机器人旋转器价格便宜且易于组装，

更新日期：2022-05-31

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