Abstract

Twenty-four-hour urine measurements play a crucial role in the diagnosis, follow-up and treatment of various diseases. There are different approaches to the collection of urine in patients who need to collect multiple urine samples at a time, especially in hospitals with heavy workloads. In this study, we compared the sodium, potassium, chloride, amylase, calcium, creatinine, phosphorus, microalbumin, protein, magnesium, urea, uric acid, adrenaline, noradrenaline, dopamine, metanephrine, normetanephrine, vanillylmandelic acid, 5-hydroxyindoleacetic acid and homovanillic acid results of 24-h urine samples analyzed immediately without acid addition, which we accepted as the reference and baseline measurement, with the results of the samples analyzed after waiting for 24 h without acid addition, analyzed immediately with acid addition and analyzed after waiting for 24 h with acid addition. Chloride, microalbumin, amylase and protein tests, which are recommended to be measured in the sample without preservatives, are affected by acid addition. Adrenaline, noradrenaline and dopamine, which are the tests recommended to be measured in acid-added urine are degraded in the samples without acid, and the levels of metanephrine and normetanephrine were not significantly degraded in the absence of preservatives.

摘要

24小时尿液测量在各种疾病的诊断、随访和治疗中起着至关重要的作用。对于需要一次采集多个尿液样本的患者，有不同的尿液采集方法，尤其是在工作量大的医院。在这项研究中，我们比较了钠、钾、氯化物、淀粉酶、钙、肌酐、磷、微量白蛋白、蛋白质、镁、尿素、尿酸、肾上腺素、去甲肾上腺素、多巴胺、变肾上腺素、去甲变肾上腺素、香草扁桃酸、5-羟基吲哚乙酸和在不加酸的情况下立即分析 24 小时尿样的高香草酸结果，我们接受其作为参考和基线测量，在不加酸的情况下等待 24 小时后分析的样品结果，加酸立即分析，加酸等待 24 h 后分析。氯化物、微量白蛋白、淀粉酶和蛋白质测试，建议在不含防腐剂的样品中进行测量，但会受到酸添加的影响。肾上腺素、去甲肾上腺素和多巴胺是推荐在加酸尿液中测量的测试，在没有酸的样品中会降解，而在没有防腐剂的情况下，变肾上腺素和去甲变肾上腺素的水平没有显着降低。