Abstract

In the maritime field where biofouling has both economic and environmental impacts, in situ quantification methods of biofilm development are of outstanding importance. Indeed, it is challenging to temporally monitor biofilm formation due to the complexity of the marine ecosystem, common inaccessibility of sampling location and lack of standardized techniques. Here, an artificial polymeric surface was tested in situ and in vitro against natural phototrophic biofilms and monoculture biofilms using plate reader fluorescence. The suitability of the developed method was verified using fluorescence microscopy coupled with image analysis - a common quantification technique - demonstrating a strong correlation between the tested methods. The results indicated the efficiency of inherent chlorophyll fluorescence in quantifying undisturbed phototrophic biofilms in field and laboratory conditions using microplate reader. This work demonstrated that the suggested approach is promising for biofilm high-throughput testing, and therefore has the potential to be used in several research and industrial sectors for monitoring phototrophic biofilm development.

摘要

在生物污损对经济和环境都有影响的海洋领域，生物膜发展的原位量化方法非常重要。事实上，由于海洋生态系统的复杂性、采样位置普遍难以接近以及缺乏标准化技术，因此对生物膜形成进行时间监测是一项挑战。在这里，人工聚合物表面在原位和体外进行了测试使用读板器荧光对抗天然光养生物膜和单一培养生物膜。使用荧光显微镜结合图像分析（一种常见的量化技术）验证了所开发方法的适用性，证明了测试方法之间的强相关性。结果表明固有叶绿素荧光在使用酶标仪在现场和实验室条件下量化未受干扰的光养生物膜方面的效率。这项工作表明，所建议的方法对于生物膜高通量测试很有前景，因此有可能在多个研究和工业部门用于监测光养生物膜的发展。