Electrospinning technique became a very common and effective method to fabricate nonwoven films with a large surface area for different purposes especially in the biomedical field. Antimicrobial submicron fibrous films based on polyvinylpyrrolidone (PVP) doped with silver nanoparticles (Ag-NPs), Zinc Oxide (ZnO), and (Ag-NPS/ZnO), were successfully fabricated via the electrospinning technique. The morphology and the elemental configurations of the as-prepared films were studied by using scanning electron microscope with EDX. While the phase formation and crystal structures were determined by using XRD analysis. The antibacterial effect was investigated against one of the most common Gram-negative bacteria Klebsiella pneumoniae by using a modified Kirby–Bauer disc diffusion method. The results showed that the doping nanoparticles were uniformly loaded on the surface of the fabricated film fibers. By using Scherrer equation the calculated average crystallite sizes of Ag-NPs, ZnO, and Ag-NPs/ZnO on PVP fibers were 63, 30, and 44 nm, respectively. The antimicrobial activity against Klebsiella pneumoniae showed the growth inhibition zones in the bacteria plates of 23.3, 54, and 60.6 mm for the samples of Ag-NPs/PVP, ZnO/PVP, and Ag-NPs/ZnO/PVP, respectively. The antimicrobial efficiency increased by forming nanocomposites of both ZnO nanoparticles and Ag nanoparticles inside the film.

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静电纺丝法制备生物活性聚乙烯吡咯烷酮薄膜及其表征

静电纺丝技术成为一种非常普遍和有效的方法来制造具有不同用途的大表面积非织造薄膜，特别是在生物医学领域。通过静电纺丝技术成功制备了基于掺杂有银纳米粒子 (Ag-NPs)、氧化锌 (ZnO) 和 (Ag-NPS/ZnO) 的聚乙烯吡咯烷酮 (PVP) 的抗菌亚微米纤维膜。采用带EDX的扫描电子显微镜对所制备薄膜的形貌和元素构型进行了研究。而相的形成和晶体结构通过使用XRD分析来确定。对一种最常见的革兰氏阴性菌肺炎克雷伯菌的抗菌作用进行了研究通过使用改进的 Kirby-Bauer 圆盘扩散法。结果表明，掺杂纳米粒子均匀地负载在制备的薄膜纤维表面。通过使用 Scherrer 方程，计算出 PVP 纤维上 Ag-NPs、ZnO 和 Ag-NPs/ZnO 的平均微晶尺寸分别为 63、30 和 44 nm。对于 Ag-NPs/PVP、ZnO/PVP 和 Ag-NPs/ZnO/PVP 样品，对肺炎克雷伯菌的抗菌活性显示，细菌平板中的生长抑制区分别为 23.3、54 和 60.6 mm。通过在薄膜内形成 ZnO 纳米颗粒和 Ag 纳米颗粒的纳米复合材料，抗菌效率得以提高。