Ophiorrhiza pumila is a medicinal plant that grows in subtropical forests and produces camptothecin (CPT). To determine an optimal harvest time of O. pumila in a plant factory with artificial light (PFAL), we investigated the CPT distribution in each organ and at the developmental stage and estimated the annual CPT production. For this study, the O. pumila plants were grown in controlled environments (16 h light period, photosynthetic photon flux density of 100 μmol m⁻² s⁻¹ under white light-emitting diode lamps, air temperature of 28 °C, relative humidity of 80%, and CO₂ concentration of 1000 μmol mol⁻¹). First, the stem, root, and seed pod had higher CPT contents than the leaves, flower, and ovary. The optimal harvest time of O. pumila in a PFAL was 63 days after transplanting (DAT), because the CPT content in the whole organs was the highest at the seed-ripening stage. Second, based on these results, the estimated annual CPT production of O. pumila cultivated in a PFAL was 380 mg m⁻² y⁻¹ (63 DAT). This value was 4.3 times greater than the annual CPT production by Camptotheca acuminata in a greenhouse. We concluded that the CPT production by O. pumila in a PFAL throughout the year has many advantages, although the demand for electrical energy was high compared to that of Camptotheca acuminata in a greenhouse.

Ophi orrhiza pumila是一种药用植物，生长在亚热带森林中，可产生喜树碱 (CPT)。为了确定人造光植物工厂 (PFAL) 中O. pumila的最佳收获时间，我们调查了每个器官和发育阶段的 CPT 分布，并估算了每年的 CPT 产量。对于这项研究，O. pumila植物生长在受控环境中（光照周期为 16 小时，光合光子通量密度为 100 μmol m ^-2  s ^-1，在白色发光二极管灯下，气温为 28 °C，相对湿度80%，CO ₂浓度为1000 μmol mol ^-1). 首先，茎、根和种子荚的 CPT 含量高于叶、花和子房。PFAL 中O. pumila的最佳收获时间是移植后 63 天 (DAT)，因为整个器官中的 CPT 含量在种子成熟阶段最高。其次，基于这些结果，在 PFAL 中种植的O. pumila的 CPT 年产量估计为 380 mg m ^-2 y ^-1 (63 DAT)。该值是喜树在温室中每年 CPT 产量的 4.3 倍。我们得出结论， O. pumila在 PFAL 中全年生产 CPT具有许多优势，尽管与温室中的喜树。