Delivery of Dissociated Islets Cells within Microporous Annealed Particle Scaffold to Treat Type 1 Diabetes,Advanced Therapeutics

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Delivery of Dissociated Islets Cells within Microporous Annealed Particle Scaffold to Treat Type 1 Diabetes
Advanced Therapeutics ( IF 3.7 ) Pub Date : 2022-07-02 , DOI: 10.1002/adtp.202200064
Colleen A Roosa ₁ , Mingyang Ma ₂ , Preeti Chhabra ₂ , Kenneth Brayman ₂ , Donald Griffin _{1,

3}

Affiliation

Type 1 diabetes (T1D) is caused by the autoimmune loss of insulin-producing beta cells in the pancreas. The only clinical approach to patient management of blood glucose that doesn't require exogenous insulin is pancreas or islet transplantation. Unfortunately, donor islets are scarce and there is substantial islet loss immediately after transplantation due, in part, to the local inflammatory response. The delivery of stem cell-derived beta cells (e.g., from induced pluripotent stem cells) and dissociated islet cells hold promise as a treatment for T1D; however, these cells typically require re-aggregation in vitro prior to implantation. Microporous scaffolds have shown high potential to serve as a vehicle for organization, survival, and function of insulin-producing cells. In this study, the use of microporous annealed particle (MAP) scaffold for delivery of enzymatically dissociated islet cells, a model beta cell source, within the scaffold's interconnected pores is investigated. It is found that MAP-based cell delivery enables survival and function of dissociated islets cells both in vitro and in an in vivo mouse model of T1D.

中文翻译：

在微孔退火颗粒支架内递送解离的胰岛细胞以治疗 1 型糖尿病

1 型糖尿病 (T1D) 是由胰腺中产生胰岛素的 β 细胞的自身免疫性丧失引起的。唯一不需要外源胰岛素的患者血糖管理临床方法是胰腺或胰岛移植。不幸的是，供体胰岛稀缺，移植后立即出现大量胰岛损失，部分原因是局部炎症反应。干细胞衍生的β细胞（例如，来自诱导多能干细胞）和分离的胰岛细胞的递送有望成为T1D的治疗方法；然而，这些细胞在植入之前通常需要在体外重新聚集。微孔支架已显示出作为胰岛素生成细胞的组织、存活和功能的载体的巨大潜力。在这项研究中，研究了使用微孔退火颗粒（MAP）支架在支架的互连孔内递送酶解的胰岛细胞（一种模型β细胞源）。研究发现，基于 MAP 的细胞递送能够使解离的胰岛细胞在体外和体内 T1D 小鼠模型中存活并发挥功能。

更新日期：2022-07-02

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