Spot blotch caused by Bipolaris sorokiniana is an economically significant disease of wheat that can cause yield losses up to 70%. It is a disease of great concern, especially in South Asia and Latin American countries, where warm-humid conditions persist during the wheat cropping season. In eastern parts of India, it is a major issue which sometimes occurs up to epidemic proportions. Lacking suitable resistant genes in high-yielding cultivars further add to the problem and reason of huge yield losses. Resistant gene analog (RGAs) are a class of resistant genes with conserved modules provides resistance against such devastating pathogens. They resist a plethora of disease-causing pathogen and orchestrated a response via activating numerous downstream cascades of pathogenesis ultimately leads to survival under biotic encounter. Here, we postulated that expression of these genes are modulated upon infection in resistant cultivar (BHU-35) compared to known susceptible cultivar i.e., Sonalika at seedling stage upon artificial infestation of two diverse isolates PUSA2 and HD3069, individually. Moreover, these sequences act as a genic marker in the cultivar and could be used to differentiate resistant from the susceptible ones. This study about RGAs genes provides a novel genic marker-assisted differential gene expression in contrasting cultivars and thus, use to generate resistance in susceptible cultivars by stacking these classes of genes using molecular breeding/biotechnological approaches. An increasing proportion of such devastating diseases in wheat-growing regions necessitated the development of resistant varieties to tackle the disease.

由Bipolaris sorokiniana引起的斑点斑是一种具有经济意义的小麦病害，可导致高达 70% 的产量损失。这是一种非常令人担忧的疾病，尤其是在南亚和拉丁美洲国家，这些国家在小麦种植季节持续存在温暖潮湿的环境。在印度东部地区，这是一个重大问题，有时会达到流行病的程度。高产品种缺乏合适的抗性基因进一步增加了产量损失巨大的问题和原因。抗性基因类似物 (RGA) 是一类具有保守模块的抗性基因，可提供对此类破坏性病原体的抗性。它们抵抗过多的致病病原体，并通过激活许多下游的发病机制来协调反应，最终导致在生物遭遇下生存。这里，我们假设这些基因的表达在抗性品种（BHU-35）的感染后受到调节，与已知的易感品种（即Sonalika）相比，在苗期分别人工侵染两种不同的分离株PUSA2和HD3069。此外，这些序列作为品种中的基因标记，可用于区分抗性和易感性。这项关于 RGAs 基因的研究在对比品种中提供了一种新的基因标记辅助差异基因表达，因此，通过使用分子育种/生物技术方法堆叠这些类别的基因，用于在易感品种中产生抗性。在小麦种植区，越来越多的此类破坏性疾病需要开发抗病品种来应对这种疾病。