General Method for Pesticide Recognition Using Albumin-Based Host–Guest Ensembles,ACS Sensors

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General Method for Pesticide Recognition Using Albumin-Based Host–Guest Ensembles
ACS Sensors ( IF 8.2 ) Pub Date : 2022-07-01 , DOI: 10.1021/acssensors.2c00803
Tianyi Qin _{1,

2} , Xiongfei Zhao ₁ , Taoyuze Lv ₃ , Guangkai Yao ₂ , Zhongyong Xu ₁ , Lei Wang ₁ , Chen Zhao ₂ , Hanhong Xu ₂ , Bin Liu ₁ , Xiaojun Peng _{1,

4}

Affiliation

The massive use of pesticides nowadays has led to serious consequences for the environment and public health. Fluorescence analytical methods for pesticides are particularly advantageous with respect to simplicity and portability; however, currently available fluorescence methods (enzyme-based assays and indicator displacement assays) with poor universality are only able to detect few specific pesticides (e.g., organophosphorus). Making use of the multiple flexible and asymmetrical binding sites in albumin, we herein report a set of multicolor albumin-based host–guest ensembles. These ensembles exhibit a universal but distinctive fluorescent response to most of the common pesticides and allow array-based identification of pesticides with high accuracy. Furthermore, the simplicity, portability, and visualization of this method enable on-site determination of pesticides in a practical setting. This albumin host strategy largely expands the toolbox of traditional indicator displacement assays (synthetic macrocycles as hosts), and we expect it to inspire a series of sensor designs for pesticide detection.

中文翻译：

使用基于白蛋白的主客体集合进行农药识别的一般方法

当今大量使用杀虫剂已对环境和公众健康造成严重后果。农药的荧光分析方法在简单性和便携性方面特别有利；然而，目前可用的荧光方法（基于酶的测定和指示剂置换测定）普遍性较差，只能检测少数特定农药（例如.，有机磷）。利用白蛋白中的多个灵活和不对称的结合位点，我们在此报告了一组基于多色白蛋白的主客体集合。这些组合对大多数常见农药表现出普遍但独特的荧光响应，并允许基于阵列的高精度农药鉴定。此外，该方法的简单性、便携性和可视化使得能够在实际环境中现场测定农药。这种白蛋白宿主策略在很大程度上扩展了传统指示剂置换分析（合成大环化合物作为宿主）的工具箱，我们希望它能够激发一系列用于农药检测的传感器设计。

更新日期：2022-07-01

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