Tomographic diffraction microscopy (TDM) is a tool of choice for high-resolution, marker-less 3D imaging of biological samples. Based on a generalization of digital holographic microscopy with full control of the sample's illumination, TDM measures, from many illumination directions, the diffracted fields in both phase and amplitude. Photon budget associated to TDM imaging is low. Therefore, TDM is not limited by phototoxicity issues. The recorded information makes it possible to reconstruct 3D refractive index distribution (with both refraction and absorption contributions) of the object under scrutiny, without any staining. In this contribution, we show an alternate use of this information. A tutorial for multimodal image reconstruction is proposed. Both intensity contrasts and phase contrasts are proposed, from the image formation model to the final reconstruction with both 2D and 3D rendering, turning TDM into a kind of ‘universal’ digital microscope.

中文翻译：

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根据断层衍射显微镜数据重建多模态图像

断层衍射显微镜 (TDM) 是生物样品高分辨率、无标记 3D 成像的首选工具。基于完全控制样品照明的数字全息显微镜的推广，TDM 从多个照明方向测量相位和振幅的衍射场。与 TDM 成像相关的光子预算较低。因此，TDM 不受光毒性问题的限制。记录的信息使得重建被观察物体的 3D 折射率分布（包括折射和吸收贡献）成为可能，而无需任何染色。在此贡献中，我们展示了此信息的另一种用途。提出了多模态图像重建教程。提出了强度对比和相位对比，