A proteolysis system, a classic biochemistry model, is proposed to enable the feasibility of indirectly assessing the quality of protein present in salmonid diets like screening system. An artificial gastric solution was developed with pepsinogen commercial, the volume of a salmonid stomach was estimated, and 8 g of food was mixed per 50 ml of solution, which was constantly stirred for 3 h and kept at 15 °C constant. We note that this artificial gastric solution requires pepsin to degrade samples, which occurs at pH 2.0, in addition, proteolysis depends on time and temperature. Comparing albumin, food and a standard mixture of proteins could observe varying degrees of fragmentation. The results suggest a low-cost system to evaluate the source of a protein, by correlated the number of fragments generated, an indicator of the quality of the protein. In addition, it allows evaluating mixtures with the disposition of the protein to be proteolyzed. The present protocol can be used for screening protein before to in vivo test and after of digestibility test or in support of digestibility protocol for improve nutrition study.

提出了一种蛋白质水解系统，一种经典的生物化学模型，以实现间接评估鲑鱼饮食中蛋白质质量的可行性，如筛选系统。用市售的胃蛋白酶原开发了一种人工胃溶液，估计鲑鱼胃的体积，每50毫升溶液混合8克食物，不断搅拌3小时，保持15℃恒定。我们注意到，这种人工胃溶液需要胃蛋白酶来降解样品，这在 pH 2.0 时发生，此外，蛋白水解取决于时间和温度。比较白蛋白、食物和标准蛋白质混合物可以观察到不同程度的碎片。结果表明，通过关联生成的片段数量（蛋白质质量的指标）来评估蛋白质来源的低成本系统。此外，它允许评估混合物与待蛋白水解的蛋白质的分布。本方案可用于在体内试验前和消化率试验后筛选蛋白质，或支持消化率方案以改善营养研究。