In the food industry, the competition and reciprocity between lactic acid bacteria (LAB) and yeasts in co-culture and the interactions of their metabolites affect product quality. Quorum sensing (QS) is a communication mechanism between bacterial cells, which participates in multiple physiological behaviors. The effects of Saccharomyces cerevisiae YE4 cell-free supernatant (CFS) on LAB were explored from the aspects of growth and metabolism, QS, and protein expression. The results showed the CFS inhibited not only the growth of Enterococcus faecium 8–3 and Lactobacillus fermentum 2-1 but also their acid production and biofilm formation. The acid production and biofilm amount was significantly decreased by 18.4% and 12.8% in L. fermentum 2–1 and E. faecium 8–3, respectively. The effect of CFS on QS was shown in the changes of Autoinducer-2 (AI-2) activity, luxS and pfs genes expression. Moreover, a total of 136 proteins of E. faecium 8-3 differed significantly in co-cultured with CFS, which involved in metabolic pathways including oxidative phosphorylation, pyruvate metabolism, metabolic pathways, mismatch repair, QS system, two-component system, and ABC transporters. Therefore, S. cerevisiae YE4 CFS might affect the physiological metabolism of E. faecium 8-3 by affecting the energy metabolism and QS system.

在食品工业中，乳酸菌 (LAB) 和酵母菌在共培养中的竞争和互惠及其代谢物的相互作用会影响产品质量。群体感应（QS）是细菌细胞之间的一种通信机制，它参与多种生理行为。从生长代谢、QS、蛋白表达等方面探讨了酿酒酵母YE4无细胞上清(CFS)对乳酸菌的影响。结果表明，CFS 不仅抑制了粪肠球菌8-3 和发酵乳杆菌2-1 的生长，而且抑制了它们的产酸和生物膜形成。发酵乳杆菌产酸量和生物膜量显着降低 18.4% 和 12.8%2-1 和E. faecium 8-3，分别。CFS 对 QS 的影响表现在 Autoinducer-2 (AI-2) 活性、luxS和pfs基因表达的变化上。此外，粪肠球菌8-3共有136种蛋白质与CFS共培养，参与代谢途径包括氧化磷酸化、丙酮酸代谢、代谢途径、错配修复、QS系统、双组分系统和ABC 运输车。因此，S. cerevisiae YE4 CFS可能通过影响能量代谢和QS系统来影响粪肠球菌8-3的生理代谢。