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Position-specific carbon isotope analysis of serine by gas chromatography/Orbitrap mass spectrometry, and an application to plant metabolism
Rapid Communications in Mass Spectrometry ( IF 1.8 ) Pub Date : 2022-06-29 , DOI: 10.1002/rcm.9347
Elise B Wilkes 1 , Alex L Sessions 1 , Sarah S Zeichner 1 , Brooke Dallas 1 , Brian Schubert 2 , A Hope Jahren 3 , John M Eiler 1
Affiliation  

Position-specific 13C/12C ratios within amino acids remain largely unexplored in environmental samples due to methodological limitations. We hypothesized that natural-abundance isotope patterns in serine may serve as a proxy for plant metabolic fluxes including photorespiration. Here we describe an Orbitrap method optimized for the position-specific carbon isotope analysis of serine to test our hypothesis and discuss the generalizability of this method to other amino acids.

中文翻译:

气相色谱/轨道阱质谱法对丝氨酸的位置特异性碳同位素分析及其在植物代谢中的应用

由于方法学的限制,氨基酸内特定位置的13 C/ 12 C 比率在环境样品中很大程度上仍未探索。我们假设丝氨酸中的天然丰度同位素模式可以作为植物代谢通量(包括光呼吸)的代表。在这里,我们描述了一种针对丝氨酸的特定位置碳同位素分析优化的 Orbitrap 方法,以检验我们的假设并讨论该方法对其他氨基酸的普遍性。
更新日期:2022-06-29
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