The alicyclic compound cyclohexane carboxylate (CHC) is anaerobically degraded through a peripheral pathway that converges with the central benzoyl-CoA degradation pathway of aromatic compounds in Rhodopseudomonas palustris (bad pathway) and some strictly anaerobic bacteria. Here we show that in denitrifying bacteria, e.g. Aromatoleum sp. CIB strain, CHC is degraded through a bad-ali pathway similar to that reported in R. palustris but that does not share common intermediates with the benzoyl-CoA degradation pathway (bzd pathway) of this bacterium. The bad-ali genes are also involved in the aerobic degradation of CHC in strain CIB, and orthologous bad-ali clusters have been identified in the genomes of a wide variety of bacteria. Expression of bad-ali genes in strain CIB is under control of the BadR transcriptional repressor, which was shown to recognize CHC-CoA, the first intermediate of the pathway, as effector, and whose operator region (CAAN₄TTG) was conserved in bad-ali clusters from Gram-negative bacteria. The bad-ali and bzd pathways generate pimelyl-CoA and 3-hydroxypimelyl-CoA, respectively, that are metabolized through a common aab pathway whose genetic determinants form a supraoperonic clustering with the bad-ali genes. A synthetic bad-ali-aab catabolic module was engineered and it was shown to confer CHC degradation abilities to different bacterial hosts.

中文翻译：

---

反硝化细菌 Aromatoleum sp. 中环己烷羧酸盐降解途径的遗传特征。商业银行

脂环族化合物环己烷羧酸酯 (CHC) 通过外周途径厌氧降解，该外周途径与沼泽红假单胞菌（不良途径）和一些严格厌氧细菌中芳香族化合物的中心苯甲酰辅酶 A 降解途径汇合。在这里，我们表明在反硝化细菌中，例如Aromatoleum sp。CIB 菌株，CHC 通过类似于R中报道的 bad-ali 途径降解。palustris但它与该细菌的苯甲酰辅酶 A 降解途径（bzd 途径）不共享共同的中间体。bad-ali基因还参与菌株 CIB 中 CHC 的有氧降解，并且直系同源bad -ali已经在多种细菌的基因组中发现了簇。菌株 CIB 中bad-ali基因的表达受 BadR 转录抑制因子的控制，该抑制因子被证明可识别 CHC-CoA（该途径的第一个中间体）作为效应子，其操纵子区域（CAAN ₄ TTG）在bad中是保守的-来自革兰氏阴性细菌的ali簇。bad-ali 和 bzd 途径分别产生庚二酰辅酶 A 和 3-羟基庚二酰辅酶 A，它们通过共同的 aab 途径代谢，其遗传决定因素与坏- ali基因形成超操纵子簇。合成的 bad-ali-aab分解代谢模块被设计出来，它被证明可以赋予不同的细菌宿主 CHC 降解能力。