This study aimed to induce osteodifferentiation by cell imprinting and beta-carotene (βC). Adipose-derived mesenchymal stem cells (ADMSCs) were seeded on MG63 cell-imprinted polydimethyl silicone (PDMS) substrate, in the presence of βC for 14 days. The MTT assay and SEM results indicated good adhesion and proliferation of ADMSCs on the imprinted substrate. Alizarin red staining, alkaline phosphate activity, and calcium assay displayed the AMSCs differentiated to ossification. Expression of ALKALINE PHOSPHATASE (ALP), COLLAGEN type I (COLL I), and OSTEOCALCIN genes approved the differentiation of ADMSCs to osteoblasts. Also, the ICC result confirmed the expression of OSTEOCALCIN protein as an osteomarker. Therefore, the PDMS cell-imprinted substrate-added βC can be used to osteogenic differentiation.

本研究旨在通过细胞印记和β-胡萝卜素（βC）诱导骨分化。在 βC 存在下，将脂肪来源的间充质干细胞 (ADMSCs) 接种在 MG63 细胞印记的聚二甲基硅氧烷 (PDMS) 基底上 14 天。MTT测定和SEM结果表明ADMSC在印迹基材上具有良好的粘附和增殖。茜素红染色、碱性磷酸盐活性和钙测定显示 AMSCs 分化为骨化。碱性磷酸酶( ALP )、I 型胶原蛋白( COLL I ) 和骨钙素的表达基因批准了 ADMSCs 向成骨细胞的分化。此外，ICC 结果证实了 OSTEOCALCIN 蛋白作为骨标志物的表达。因此，PDMS细胞印迹底物添加的βC可用于成骨分化。