Adoptive cell therapy using umbilical cord blood (UCB)-derived allogeneic natural killer (NK) cells has shown encouraging results. However, because of the insufficient availability of NK cells and limited UCB volume, more effective culture methods are required. NK cell expansion and functionality are largely affected by the culture medium. While human serum is a major affecting component in culture media, the way it regulates NK cell functionality remains elusive. We elucidated the effects of different culture media and human serum supplementation on UCB NK cell expansion and functionality.

中文翻译：

---

自然杀伤细胞扩增和细胞毒性因使用的培养基而异。

使用脐带血 (UCB) 衍生的同种异体自然杀伤 (NK) 细胞的过继细胞疗法已显示出令人鼓舞的结果。然而，由于 NK 细胞的可用性不足和 UCB 体积有限，需要更有效的培养方法。NK 细胞的扩增和功能在很大程度上受培养基的影响。虽然人血清是培养基中的主要影响成分，但它调节 NK 细胞功能的方式仍然难以捉摸。我们阐明了不同培养基和人血清补充剂对 UCB NK 细胞扩增和功能的影响。