Frontiers in Ecology and Evolution ( IF 2.4 ) Pub Date : 2022-06-30 , DOI: 10.3389/fevo.2022.910009 Stefanie Agne , Gavin J. P. Naylor , Michaela Preick , Lei Yang , Ralf Thiel , Simon Weigmann , Johanna L. A. Paijmans , Axel Barlow , Michael Hofreiter , Nicolas Straube
Etmopteridae (lantern sharks) is the most species-rich family of sharks, comprising more than 50 species. Many species are described from few individuals, and re-collection of specimens is often hindered by the remoteness of their sampling sites. For taxonomic studies, comparative morphological analysis of type specimens housed in natural history collections has been the main source of evidence. In contrast, DNA sequence information has rarely been used. Most lantern shark collection specimens, including the types, were formalin fixed before long-term storage in ethanol solutions. The DNA damage caused by both fixation and preservation of specimens has excluded these specimens from DNA sequence-based phylogenetic analyses so far. However, recent advances in the field of ancient DNA have allowed recovery of wet-collection specimen DNA sequence data. Here we analyse archival mitochondrial DNA sequences, obtained using ancient DNA approaches, of two wet-collection lantern shark paratype specimens, namely
中文翻译:
基于来自湿采集副型的线粒体 DNA 对两个鲜为人知的灯笼鲨物种的分类学鉴定
Etmopteridae(灯笼鲨)是物种最丰富的鲨鱼科,包括 50 多种。许多物种是从少数个体中描述的,并且样本的重新收集通常受到其采样地点偏远的阻碍。对于分类学研究,自然历史收藏中类型标本的比较形态分析一直是主要的证据来源。相比之下,很少使用 DNA 序列信息。大多数灯笼鲨收集标本,包括这些类型,在长期储存在乙醇溶液中之前都经过福尔马林固定。迄今为止,由于标本的固定和保存引起的 DNA 损伤已将这些标本排除在基于 DNA 序列的系统发育分析之外。然而,古代 DNA 领域的最新进展已经允许恢复湿收集标本 DNA 序列数据。