Gold nanoparticle-based lateral flow immunoassay for the rapid detection of flumetralin in orange,Analyst

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Gold nanoparticle-based lateral flow immunoassay for the rapid detection of flumetralin in orange
Analyst ( IF 3.6 ) Pub Date : 2022-06-30 , DOI: 10.1039/d2an00899h
Xinxin Xu _{1,

2} , Lu Lin _{1,

2} , Hua Kuang _{1,

2} , Liqiang Liu _{1,

2} , Liguang Xu _{1,

2} , Chuanlai Xu _{1,

2}

Affiliation

Residues of flumetralin, a dinitroaniline herbicide, in foods pose a health risk to humans. In this study, we produced a sensitive monoclonal antibody (mAb) against flumetralin and developed a gold nanoparticle-based lateral flow immunoassay (LFIA) strip for screening flumetralin in orange. The subtype of mAb 3F5 was IgG1 and had a half-maximal inhibitory concentration of 9.43 ng mL⁻¹. The cross-reactivity assay indicated that mAb had good specificity. The LFIA strip had a visual limit of detection of 200 ng g⁻¹ in orange. We obtained results with the naked eye within 15 min. The calculated LOD of the strip in orange was 33.26 ng g⁻¹, with a linear detection of 80.72–1672.10 ng g⁻¹. The strip had recovery rates ranging from 98.4% to 110.1%. Therefore, our developed method is an effective and reliable tool for the rapid detection of flumetralin in orange.

中文翻译：

基于金纳米粒子的横向流动免疫分析法快速检测橙中氟美曲林

食品中的氟美曲林（一种二硝基苯胺类除草剂）的残留物对人类健康构成威胁。在这项研究中，我们生产了一种针对氟美曲林的敏感单克隆抗体 (mAb)，并开发了一种基于金纳米颗粒的横向流动免疫测定 (LFIA) 条带，用于筛选橙色中的氟美曲林。mAb 3F5的亚型是IgG1，其半数最大抑制浓度为9.43 ng mL ^-1。交叉反应性测定表明mAb具有良好的特异性。LFIA 试纸条的橙色检测限为 200 ng g ^-1。我们在 15 分钟内用肉眼获得了结果。橙色条带的计算 LOD 为 33.26 ng g ^-1，线性检测为 80.72–1672.10 ng g ^-1. 条带的回收率在 98.4% 到 110.1% 之间。因此，我们开发的方法是快速检测橙中氟美曲林的有效且可靠的工具。

更新日期：2022-06-30

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