CdpNPT from Aspergillus fumigatus is a fungal indole prenyltransferase (IPT) with remarkable substrate promiscuity to generate prenylated compounds. Our first investigation of the catalytic potential of CdpNPT against a β-carboline, harmol (1), revealed that the enzyme also accepts 1 as the prenyl acceptor with dimethylallyl diphosphate (DMAPP) as the prenyl donor and selectively prenylates the C-6 position of 1 by the “regular-type” dimethylallylation to produce 6-(3-dimethylallyl)harmol (2). Furthermore, our X-ray crystal structure analysis of the C-His₆-tagged CdpNPT (38-440) truncated mutant complexed with 1 and docking studies of DMAPP to the crystal structure of the CdpNPT (38-440) mutant suggested that CdpNPT could employ the two-step prenylation system to produce 2.

来自烟曲霉的CdpNPT是一种真菌吲哚异戊二烯转移酶 (IPT)，具有显着的底物混杂性以产生异戊二烯化化合物。我们对 CdpNPT 对β-咔啉、harmol ( 1 ) 的催化潜力的首次研究表明，该酶还接受1作为异戊二烯受体，二甲基烯丙基二磷酸 (DMAPP) 作为异戊二烯供体并选择性异戊二烯化的 C-6 位置1通过“常规型”二甲基烯丙基化生成 6-(3-二甲基烯丙基)harmol ( 2 )。此外，我们对与1复合的 C-His ₆标记的​​ CdpNPT (38-440) 截短突变体的 X 射线晶体结构分析DMAPP 与 CdpNPT (38-440) 突变体晶体结构的对接研究表明，CdpNPT 可以采用两步异戊二烯化系统生产2。