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Solid phase extraction on reverse phase chromatographic media subjected to stresses expected for extraterrestrial implementation
Analyst ( IF 3.6 ) Pub Date : 2022-06-29 , DOI: 10.1039/d2an00768a Jerome P Ferrance 1
Analyst ( IF 3.6 ) Pub Date : 2022-06-29 , DOI: 10.1039/d2an00768a Jerome P Ferrance 1
Affiliation
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Sample preparation techniques, such as solid phase extraction, will likely be required for in situ analysis of liquid samples collected from bodies in our Solar System that contain liquid, to concentration and desalt analytes of interest from the expected brines on these Ocean Worlds. Media to be used for these extraction procedures will have to survive the stresses of the long spaceflight required to reach these bodies, and remain functional once at that location. This work utilized tryptophan as an initial representative analyte to evaluate capture and desalting efficiencies in silica and polymeric reverse phase media, to determine how these solid phases might withstand stresses they could experience during deployment, including vacuum exposure, freezing, and heating/sonication treatments. Further experimentation on irradiation and long term freezing of media with an expanded array of analytes evaluated the utility of reverse phase media for this application. Kromasil® C-18 silica particles performed well, showing no loss in capture or desalting efficiency for the initial stress treatments or irradiation, but long term freezing after irradiation caused issues with this media. Oasis® HLB polymeric particles performed better, with 100% capture efficiency and 90% recovery of the tryptophan analyte for all treated and the untreated media. Onyx C-18 guard cartridges, a reverse phase C-18 modified silica monolithic media, exhibiting 100% capture efficiency and >90% recovery of tryptophan for both untreated and treated monoliths but also had issues after irradiation and long term frozen storage. Chromolith® RP-18e silica monolithic guard cartridges showed issues with consistency and reproducibility. In expanding the list of analytes, the Oasis® HLB media showed the best performance, capturing more of the analytes tested and remaining fully functional through both irradiation and long term storage treatments. Other media with additional reverse phase capture characteristics were also evaluated but none performed as well on the selected analytes as the Oasis® HLB media.
中文翻译：
反相色谱介质上的固相萃取受到外星实施预期的应力
原位可能需要样品制备技术，例如固相萃取分析从我们太阳系中的物体收集的含有液体的液体样品，以浓缩和脱盐这些海洋世界上预期的盐水中的感兴趣的分析物。用于这些提取程序的介质必须能够承受到达这些物体所需的长途太空飞行的压力，并在该位置保持功能。这项工作利用色氨酸作为初始代表性分析物来评估二氧化硅和聚合物反相介质中的捕获和脱盐效率，以确定这些固相如何承受它们在部署过程中可能遇到的压力，包括真空暴露、冷冻和加热/超声处理。对具有扩展的分析物阵列的介质进行辐照和长期冷冻的进一步实验评估了反相介质在该应用中的效用。Kromasil® C-18 二氧化硅颗粒表现良好，在初始应力处理或辐照中没有显示捕获或脱盐效率的损失，但辐照后的长期冻结会导致该介质出现问题。Oasis® HLB 聚合物颗粒表现更好，对所有处理和未处理的介质具有 100% 的捕获效率和 90% 的色氨酸分析物回收率。Onyx C-18 保护柱，一种反相 C-18 改性二氧化硅整体介质，对于未经处理和处理的整体而言，表现出 100% 的捕获效率和 > 90% 的色氨酸回收率，但在辐照和长期冷冻储存后也存在问题。Chromolith® RP-18e 二氧化硅整体保护柱显示出一致性和重现性问题。在扩展分析物列表时，Oasis® HLB 介质表现出最佳性能，可捕获更多被测分析物，并通过辐照和长期储存处理保持完整功能。还评估了具有额外反相捕获特性的其他介质，但没有一种介质在选定分析物上的表现不如 Oasis® HLB 介质。
更新日期：2022-06-29
中文翻译：
反相色谱介质上的固相萃取受到外星实施预期的应力
原位可能需要样品制备技术，例如固相萃取分析从我们太阳系中的物体收集的含有液体的液体样品，以浓缩和脱盐这些海洋世界上预期的盐水中的感兴趣的分析物。用于这些提取程序的介质必须能够承受到达这些物体所需的长途太空飞行的压力，并在该位置保持功能。这项工作利用色氨酸作为初始代表性分析物来评估二氧化硅和聚合物反相介质中的捕获和脱盐效率，以确定这些固相如何承受它们在部署过程中可能遇到的压力，包括真空暴露、冷冻和加热/超声处理。对具有扩展的分析物阵列的介质进行辐照和长期冷冻的进一步实验评估了反相介质在该应用中的效用。Kromasil® C-18 二氧化硅颗粒表现良好，在初始应力处理或辐照中没有显示捕获或脱盐效率的损失，但辐照后的长期冻结会导致该介质出现问题。Oasis® HLB 聚合物颗粒表现更好，对所有处理和未处理的介质具有 100% 的捕获效率和 90% 的色氨酸分析物回收率。Onyx C-18 保护柱，一种反相 C-18 改性二氧化硅整体介质，对于未经处理和处理的整体而言，表现出 100% 的捕获效率和 > 90% 的色氨酸回收率，但在辐照和长期冷冻储存后也存在问题。Chromolith® RP-18e 二氧化硅整体保护柱显示出一致性和重现性问题。在扩展分析物列表时，Oasis® HLB 介质表现出最佳性能，可捕获更多被测分析物，并通过辐照和长期储存处理保持完整功能。还评估了具有额外反相捕获特性的其他介质，但没有一种介质在选定分析物上的表现不如 Oasis® HLB 介质。
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