Microbial volatile organic compounds (VOCs) are effective biofumigants for controlling postharvest fruit decay. We evaluated the postharvest biofumigation efficacy of the VOCs produced by Burkholderia cenocepacia ETR-B22 for alleviating tomato fruit grey mould and its impact on the fruit quality. The results demonstrated that the VOCs inhibited the hyphal growth and spore germination of pathogen Botrytis cinerea and caused hyphal cell death. Postharvest biofumigation with VOCs significantly decreased disease incidence, disease index, and weight loss of tomato fruit. The antifungal efficacy of the VOCs was affected by growth media substrates, inoculation volume, and concentration. Among the analysed VOC components, dimethyl trisulfide was most effective in inhibiting B. cinerea. Biofumigation with VOCs produced by ETR-B22 delayed the deterioration of the nutrition quality of tomato fruit. Metabolomics analysis revealed that the aromatic metabolite profile of postharvest tomato fruit was well-maintained after fumigation with ETR-B22 VOCs. Therefore, B. cenocepacia ETR-B22 VOCs could be used as useful biofumigants for extending postharvest tomato fruit shelf life and controlling grey mould disease.

微生物挥发性有机化合物 (VOC) 是控制采后果实腐烂的有效生物熏蒸剂。我们评估了新洋葱伯克霍尔德菌ETR-B22产生的 VOC 对缓解番茄果实灰霉病的采后生物熏蒸效果及其对果实品质的影响。结果表明，VOCs抑制病原灰霉病菌的菌丝生长和孢子萌发，导致菌丝细胞死亡。用 VOCs 进行采后生物熏蒸显着降低了番茄果实的发病率、疾病指数和重量损失。VOCs 的抗真菌功效受生长培养基底物、接种量和浓度的影响。在所分析的 VOC 成分中，二甲基三硫化物对抑制B.灰霉病。ETR-B22产生的VOCs生物熏蒸延缓了番茄果实营养品质的恶化。代谢组学分析表明，采后番茄果实的芳香代谢物特征在用 ETR-B22 VOCs 熏蒸后得到很好的保持。因此，B. cenocepacia ETR-B22 VOCs 可用作延长采后番茄果实保质期和控制灰霉病的有用生物熏蒸剂。