Procedures for chitin nanofibers extraction from mushroom significantly modify their structure and physicochemical properties, through disintegration and surface oxidation of glucan residue, as well as surface deacetylation of chitin. Here, four kinds of chitin-glucan nanofibers (CGNF) were isolated form Shiitake stipes via different alkali treatment conditions, wherein glucan content ranged from 6.4 % to 46.8 %. Observations with transmission electron microscopy showed that CGNFs possessed average widths with 5.1 ± 1.2 to 7.1 ± 1.5 nm. The glucan showed a negative effect on the crystal index and thermal stability of CGNFs. A strong positive correlation was observed between glucan residues and zeta potential value. The phenomenon about the increase of viscosity, yield stress and elastic modulus upon glucan decrease was discussed. Overall, the residual glucan offers fungi-derived chitin nanomaterials a diversity of material properties and tuning its content is a feasible approach for customize nano chitin fibers used in nutraceutical and food industry.

通过葡聚糖残基的分解和表面氧化以及几丁质的表面脱乙酰化，从蘑菇中提取几丁质纳米纤维的程序显着改变了它们的结构和物理化学性质。在这里，通过不同的碱处理条件从香菇柄中分离出四种甲壳素-葡聚糖纳米纤维（CGNF），其中葡聚糖含量在6.4%到46.8%之间。透射电子显微镜观察表明，CGNF 的平均宽度为 5.1 ± 1.2 至 7.1 ± 1.5 nm。葡聚糖对CGNFs的晶体指数和热稳定性有负面影响。在葡聚糖残基和zeta电位值之间观察到强正相关。讨论了葡聚糖减少时粘度、屈服应力和弹性模量增加的现象。全面的，