Ultrasensitive Detection of Exosomes Using an Optical Microfiber Decorated with Plasmonic MoSe2-Supported Gold Nanorod Nanointerfaces,ACS Sensors

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Ultrasensitive Detection of Exosomes Using an Optical Microfiber Decorated with Plasmonic MoSe2-Supported Gold Nanorod Nanointerfaces
ACS Sensors ( IF 8.2 ) Pub Date : 2022-06-27 , DOI: 10.1021/acssensors.2c00598
Hongtao Li _{1,

2,

3} , Tianqi Huang _{1,

2} , Liang Lu _{1,

2} , Hao Yuan _{1,

2} , Lei Zhang _{1,

2} , Hongzhi Wang _{4,

5} , Benli Yu _{1,

2}

Affiliation

Exosomes are potential and promising natural noninvasive biomarkers for liquid biopsies and can be involved in various biological and pathological processes in early-stage cancer. Thus, there is an urgent demand to develop low-cost, small-size, remarkable-specificity, and ultrasensitive exosome biosensors for early clinical point-of-care (POC) testing. Although various conventional tumor exosome detection methods have been generally proposed, the low detection sensitivity and specificity significantly hinder their use in cancer clinical diagnosis and prognosis. To address the above challenges, an optical microfiber integrated with MoSe₂-supported gold nanorods is proposed. To tune the strong localized surface plasmon resonance (LSPR) of the nanointerfaces on the optical microfiber to be in accordance with the operating wavelength of the silica optical microfiber in the telecommunication band, gold nanorods with a high aspect ratio of approximately 10:1 are proposed. Due to the interaction between the excited LSPR effect and the evanescent field of the optical microfiber, the sensor can detect clear cell renal cancer exosomes within a wide concentration range from 10⁰ particles/mL to 10⁸ particles/mL, with an extremely low limit of detection (LOD) of 9.32 particles/mL, which is lower than that of current various state of the art methods. More importantly, the microfiber with high specificity can successfully differentiate pathological plasma and healthy controls, exhibiting very promising clinical applications in renal cancer diagnosis and prognosis. This work opens up a new approach for the in situ detection and quantification of exosomes with ultrahigh sensitivity in early clinical screening and diagnosis.

中文翻译：

使用装饰有等离子体 MoSe2 支持的金纳米棒纳米界面的光学微纤维对外泌体进行超灵敏检测

外泌体是用于液体活检的潜在且有前景的天然非侵入性生物标志物，可参与早期癌症的各种生物和病理过程。因此，迫切需要开发用于早期临床即时 (POC) 测试的低成本、小尺寸、显着特异性和超灵敏的外泌体生物传感器。尽管已经普遍提出了各种常规的肿瘤外泌体检测方法，但检测灵敏度和特异性低，严重阻碍了它们在癌症临床诊断和预后中的应用。为了应对上述挑战，一种与 MoSe _{2集成的光纤}提出了支持的金纳米棒。为了将光纤上纳米界面的强局域表面等离子体共振 (LSPR) 调整为与通信波段中二氧化硅光纤的工作波长一致，提出了具有大约 10:1 的高纵横比的金纳米棒. ^{由于激发的 LSPR 效应和光学微纤维的渐逝场之间的相互作用，该传感器可以在从 10 0}粒子/mL 到 10 ⁸的宽浓度范围内检测透明细胞肾癌外泌体颗粒/mL，检测限 (LOD) 极低，为 9.32 个颗粒/mL，低于当前各种最先进方法的检测限。更重要的是，具有高特异性的超细纤维可以成功区分病理血浆和健康对照，在肾癌诊断和预后中展现出非常有前景的临床应用。这项工作为在早期临床筛查和诊断中以超高灵敏度原位检测和定量外泌体开辟了一条新途径。

更新日期：2022-06-27

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