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Tandem Mass Spectrometry for Structural Characterization of Doubly-Charged N-Linked Glycopeptides
ACS Environmental Au ( IF 6.7 ) Pub Date : 2022-06-28 , DOI: 10.1021/jasms.2c00143 H.-T. Kitty Wong 1 , Xiangfeng Chen 1, 2 , Simin Zhang 1 , T.-Y Lui 1 , D. Hu 1 , T.-W. Dominic Chan 1
ACS Environmental Au ( IF 6.7 ) Pub Date : 2022-06-28 , DOI: 10.1021/jasms.2c00143 H.-T. Kitty Wong 1 , Xiangfeng Chen 1, 2 , Simin Zhang 1 , T.-Y Lui 1 , D. Hu 1 , T.-W. Dominic Chan 1
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Three dissociation methods, including collision-induced dissociation (CID), electron capture dissociation (ECD), and electronic excitation dissociation (EED), were systematically compared for structural characterization of doubly charged glycopeptide. CID produced distinctively different tandem mass spectra for glycopeptide adducted with different charge carriers. Protonated species produced mainly glycosidic cleavages in high abundance. CID of magnesiated glycopeptide formed more cross-ring cleavages, whereas doubly sodiated species produced cleavages at both glycan and peptide moieties. The effect of charge carriers on the fragmentation in ECD and EED was lower than that in CID. ECD produced mainly peptide backbone cleavages but limited cleavages at the glycan moiety, whereas EED of glycopeptide resulted in extensive fragmentation throughout the molecular ion regardless of the charge carriers. Magnesiated species gave, however, more cross-ring cleavages than other charge carriers did. These results demonstrated that EED of magnesiated species could be used as a one-step dissociation method for comprehensive structural analysis of glycopeptides.
中文翻译：
串联质谱法用于双电荷 N-连接糖肽的结构表征
系统比较了三种解离方法，包括碰撞诱导解离 (CID)、电子捕获解离 (ECD) 和电子激发解离 (EED)，用于双电荷糖肽的结构表征。CID 为与不同电荷载体加合物的糖肽产生了明显不同的串联质谱。质子化的物种主要产生高丰度的糖苷切割。镁化糖肽的 CID 形成更多的交叉环切割，而双钠化物种在聚糖和肽部分均产生切割。电荷载流子对 ECD 和 EED 碎裂的影响低于 CID。ECD 主要产生肽骨架切割，但在聚糖部分产生有限的切割，而糖肽的 EED 会导致整个分子离子的广泛碎裂，而与电荷载体无关。然而，镁化物质比其他电荷载流子产生更多的交叉环劈裂。这些结果表明，镁化物质的 EED 可用作糖肽综合结构分析的一步解离方法。
更新日期：2022-06-28
中文翻译：
串联质谱法用于双电荷 N-连接糖肽的结构表征
系统比较了三种解离方法，包括碰撞诱导解离 (CID)、电子捕获解离 (ECD) 和电子激发解离 (EED)，用于双电荷糖肽的结构表征。CID 为与不同电荷载体加合物的糖肽产生了明显不同的串联质谱。质子化的物种主要产生高丰度的糖苷切割。镁化糖肽的 CID 形成更多的交叉环切割，而双钠化物种在聚糖和肽部分均产生切割。电荷载流子对 ECD 和 EED 碎裂的影响低于 CID。ECD 主要产生肽骨架切割，但在聚糖部分产生有限的切割，而糖肽的 EED 会导致整个分子离子的广泛碎裂，而与电荷载体无关。然而，镁化物质比其他电荷载流子产生更多的交叉环劈裂。这些结果表明，镁化物质的 EED 可用作糖肽综合结构分析的一步解离方法。
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