Highly accurate multiprotein detection on a digital ELISA platform,Lab on a Chip

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Highly accurate multiprotein detection on a digital ELISA platform
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-06-27 , DOI: 10.1039/d2lc00388k
Shih-Mo Yang ₁ , Qingbo Bi ₁ , Wen Jun Zhang ₂ , Xiaochuan Cui ₃ , Ying Zhou ₄ , Cunyin Yuan ₅ , Yubao Cui ₅

Affiliation

The emerging single-molecule detection platform digital enzyme-linked immunosorbent assay (ELISA) can detect numerous proteins simultaneously at serum concentrations as low as picograms per milliliter. We sought to improve cytokine detection with this platform to aid diagnosis of conditions such as allergy and asthma. We developed a multiple single-molecule detection digital ELISA approach, through the application of encoded magnetic microbeads to simultaneously detect three cytokines in one serum sample. We tested the approach's utility to distinguish asthma-related cytokines in children. Concentrations of interleukin-4 (IL-4) and IL-6 were significantly higher in children with asthma than in healthy controls, while the concentration of interferon-γ (IFN-γ) was significantly lower. Our method has higher accuracy than conventional methods, and our results indicate that the proposed improved high-sensitivity digital ELISA-based diagnosis approach can facilitate early detection and treatment of childhood asthma or related diseases.

中文翻译：

在数字 ELISA 平台上进行高度准确的多蛋白检测

新兴的单分子检测平台数字酶联免疫吸附试验（ELISA）可以同时检测血清浓度低至皮克/毫升的多种蛋白质。我们试图通过该平台改进细胞因子检测，以帮助诊断过敏和哮喘等疾病。我们开发了一种多单分子检测数字 ELISA 方法，通过应用编码的磁性微珠同时检测一个血清样本中的三种细胞因子。我们测试了该方法在区分儿童哮喘相关细胞因子方面的效用。哮喘患儿的白细胞介素 4 (IL-4) 和 IL-6 浓度显着高于健康对照组，而干扰素-γ (IFN-γ) 浓度显着降低。我们的方法比传统方法具有更高的准确性，

更新日期：2022-06-27

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