Super-resolution imaging techniques that overcome the diffraction limit of light have gained wide popularity for visualizing cellular structures with nanometric resolution. Following the pace of hardware developments, the availability of new fluorescent probes with superior properties is becoming ever more important. In this context, fluorescent nanoparticles (NPs) have attracted increasing attention as bright and photostable probes that address many shortcomings of traditional fluorescent probes. The use of NPs for super-resolution imaging is a recent development and this provides the focus for the current review. We give an overview of different super-resolution methods and discuss their demands on the properties of fluorescent NPs. We then review in detail the features, strengths, and weaknesses of each NP class to support these applications and provide examples from their utilization in various biological systems. Moreover, we provide an outlook on the future of the field and opportunities in material science for the development of probes for multiplexed subcellular imaging with nanometric resolution.

中文翻译：

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用于超分辨率成像的荧光纳米颗粒


克服光衍射极限的超分辨率成像技术在以纳米分辨率可视化细胞结构方面获得了广泛的应用。随着硬件发展的步伐，具有优异性能的新型荧光探针的可用性变得越来越重要。在这种背景下，荧光纳米粒子（NP）作为明亮且耐光的探针，解决了传统荧光探针的许多缺点，引起了越来越多的关注。使用纳米粒子进行超分辨率成像是最近的发展，这为当前综述提供了重点。我们概述了不同的超分辨率方法，并讨论了它们对荧光纳米粒子性能的要求。然后，我们详细回顾每个 NP 类别的特征、优点和缺点，以支持这些应用，并提供它们在各种生物系统中的利用示例。此外，我们还对该领域的未来进行了展望，并提供了材料科学中用于开发纳米​​分辨率多重亚细胞成像探针的机会。