Donkey milk (DM), similar to human milk (HM) in chemical composition, has been suggested as the best potential hypoallergenic replacement diet for babies suffering from Cow milk (CM) protein allergy. In order to better understand DM protein, many studies based on proteomic have been performed. In this study, the label-free quantitative proteomic approach was conducted to quantitatively identify the differentially expressed whey proteins (DEPs) in DM vs. HM group and DM vs. CM group. In total, 241 and 365 DEPs were found in these two groups, respectively. Bioinformatics analysis of DEPs showed that the majority of DEPs participated in the lipoprotein metabolic process, regulation of cytokine production, chemical homeostasis, and catabolic process. The Kyoto Encyclopedia of Gene and Genomes (KEGG) pathways analysis found that these DEPs mainly participated in an antigen processing, complement, and coagulation cascades. These results may provide valuable information in the composition of milk whey proteins in DM, HM, and CM, especially for low abundant components, and expand our knowledge of different biological functions between DM and HM or CM.

驴奶 (DM) 在化学成分上与人奶 (HM) 相似，已被建议作为患有牛奶 (CM) 蛋白质过敏婴儿的最佳潜在低过敏性替代饮食。为了更好地了解 DM 蛋白，已经进行了许多基于蛋白质组学的研究。在本研究中，采用无标记定量蛋白质组学方法定量鉴定 DM 与 HM 组和 DM 与 CM 组中差异表达的乳清蛋白 (DEP)。在这两组中，总共分别发现了 241 和 365 个 DEP。DEPs的生物信息学分析表明，大多数DEPs参与脂蛋白代谢过程、细胞因子产生的调节、化学稳态和分解代谢过程。京都基因和基因组百科全书 (KEGG) 通路分析发现，这些 DEP 主要参与抗原加工、补体和凝血级联反应。这些结果可能为 DM、HM 和 CM 中乳清蛋白的组成提供有价值的信息，特别是对于低丰度成分，并扩展我们对 DM 和 HM 或 CM 之间不同生物学功能的认识。