Diabetes kidney damage (DKD) is a chronic inflammatory disease of the kidney induced with continuous hyperglycemia as the most prevalent consequence of diabetes. Washingtonia filifera seed oil (WFO) was used as a traditional medicine to cure various diseases in ancient Saudi. This work was carried out to investigate the potential protective impact of WFO against DKD on streptozotocin (STZ)-induced type 2 diabetic mice (C57BL/6 mice). The mice were randomly split into groups: C, C + WFO (200 mg/Kg B.W.), T2D, and T2D + WFO (200 mg/Kg B.W.). Diabetes was created in mice groups except for the control group after 6 weeks of high-fat diet (HFD) feeding. Treatments with STZ (60 mg/kg body weight) were administered three times for 6 weeks, and after that, mice were sacrificed. Kidney tissues and serum were obtained to analyze levels of insulin, metabolism of lipids [triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and free fatty acids (FFA)], antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)], creatine, and blood urea nitrogen (BUN). In addition, H&E staining had been used to investigate the histological changes of the kidneys. In T2D mice, WFO corrected aberrant serum lipids (TG, TC, HDL, LDL, and FFA), elevated antioxidative enzyme levels (CAT, SOD, and GPx), and inhibited GST to various degrees. In addition, WFO improves kidney pathological traits such as fibrosis of the kidney, hypertrophy of glomeruli, and basement membrane thickness of glomeruli. Through hypoglycemic, hypolipidemic, antioxidative, and anti-inflammatory actions, WFO might ameliorate diabetic alterations in T2D mice. WFO could significantly reduce AGE buildup in the T2D mice kidneys, therefore alleviating kidney oxidative stress and inflammatory kidney damage.

中文翻译：

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Washingtonia filifera 油对 STZ 诱导的糖尿病小鼠肾脏的保护和促进健康的影响,Washingtonia filifera 油对 STZ 诱导的糖尿病小鼠肾脏的保护和促进健康的影响

糖尿病肾损害 (DKD) 是一种由持续高血糖引起的肾脏慢性炎症性疾病，是糖尿病最普遍的后果。Washingtonia filifera 种子油 (WFO) 在古代沙特被用作治疗各种疾病的传统药物。这项工作旨在研究 WFO 对 DKD 对链脲佐菌素 (STZ) 诱导的 2 型糖尿病小鼠 (C57BL/6 小鼠) 的潜在保护作用。将小鼠随机分成组：C、C + WFO (200 mg/Kg BW)、T2D 和 T2D + WFO (200 mg/Kg BW)。在高脂饮食 (HFD) 喂养 6 周后，除对照组外的小鼠组均出现糖尿病。STZ (60 mg/kg 体重) 治疗 3 次，持续 6 周，然后处死小鼠。获得肾组织和血清以分析胰岛素水平，脂质代谢 [甘油三酯 (TG)、总胆固醇 (TC)、高密度脂蛋白 (HDL)、低密度脂蛋白 (LDL) 和游离脂肪酸 (FFA)]、抗氧化酶 [超氧化物歧化酶 (SOD)、过氧化氢酶(CAT) 和谷胱甘肽过氧化物酶 (GPx)]、肌酸和血尿素氮 (BUN)。此外，H&E 染色已用于研究肾脏的组织学变化。在 T2D 小鼠中，WFO 纠正了异常的血脂（TG、TC、HDL、LDL 和 FFA），提高了抗氧化酶水平（CAT、SOD 和 GPx），并在不同程度上抑制了 GST。此外，WFO可改善肾脏病理特征，如肾脏纤维化、肾小球肥大、肾小球基底膜厚度等。通过降血糖、降血脂、抗氧化和抗炎作用，WFO 可能会改善 T2D 小鼠的糖尿病改变。WFO 可以显着减少 T2D 小鼠肾脏中的 AGE 积聚，从而减轻肾脏氧化应激和炎症性肾脏损伤。,糖尿病肾损害 (DKD) 是一种由持续高血糖引起的肾脏慢性炎症性疾病，是糖尿病最普遍的后果。Washingtonia filifera 种子油 (WFO) 在古代沙特被用作治疗各种疾病的传统药物。这项工作旨在研究 WFO 对 DKD 对链脲佐菌素 (STZ) 诱导的 2 型糖尿病小鼠 (C57BL/6 小鼠) 的潜在保护作用。将小鼠随机分成组：C、C + WFO (200 mg/Kg BW)、T2D 和 T2D + WFO (200 mg/Kg BW)。在高脂饮食 (HFD) 喂养 6 周后，除对照组外的小鼠组均出现糖尿病。STZ (60 mg/kg 体重) 治疗 3 次，持续 6 周，然后处死小鼠。获得肾组织和血清以分析胰岛素水平，脂质代谢 [甘油三酯 (TG)、总胆固醇 (TC)、高密度脂蛋白 (HDL)、低密度脂蛋白 (LDL) 和游离脂肪酸 (FFA)]、抗氧化酶 [超氧化物歧化酶 (SOD)、过氧化氢酶(CAT) 和谷胱甘肽过氧化物酶 (GPx)]、肌酸和血尿素氮 (BUN)。此外，H&E 染色已用于研究肾脏的组织学变化。在 T2D 小鼠中，WFO 纠正了异常的血脂（TG、TC、HDL、LDL 和 FFA），提高了抗氧化酶水平（CAT、SOD 和 GPx），并在不同程度上抑制了 GST。此外，WFO可改善肾脏病理特征，如肾脏纤维化、肾小球肥大、肾小球基底膜厚度等。通过降血糖、降血脂、抗氧化和抗炎作用，WFO 可能会改善 T2D 小鼠的糖尿病改变。WFO 可以显着减少 T2D 小鼠肾脏中的 AGE 积聚，从而减轻肾脏氧化应激和炎症性肾脏损伤。