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A novel inovirus reprograms metabolism and motility of marine Alteromonas
bioRxiv - Microbiology Pub Date : 2022-06-23 , DOI: 10.1101/2022.06.23.497286 Kuntong Jia , Yongyi Peng , Xueji Chen , Huahua Jian , Min Jin , ZhiWei Yi , Ming Su , Xiyang Dong , Meisheng Yi
bioRxiv - Microbiology Pub Date : 2022-06-23 , DOI: 10.1101/2022.06.23.497286 Kuntong Jia , Yongyi Peng , Xueji Chen , Huahua Jian , Min Jin , ZhiWei Yi , Ming Su , Xiyang Dong , Meisheng Yi
Members from the Inoviridae family with striking features are widespread, highly diverse and ecologically pervasive across multiple hosts and environments; however, very small amount of inoviruses have been isolated and studied. Here, a filamentous phage infecting Alteromonas abrolhosensis, designated ΦAFP1, was isolated from the South China Sea and represented as a novel genus of Inoviridae. ΦAFP1 consisted of a single-stranded DNA genome (5986 bp), encoding eight putative ORFs. Comparative analyses revealed ΦAFP1 could be regarded as genetic mosaics, which especially came from Ralstonia and Stenotrophomonas phages. The temporal transcriptome analysis of A. abrolhosensis to ΦAFP1 infectionreveals that 7.78% of the host genes were differentially expressed. The genes involved in translation processes, ribosome pathways and degradation of multiple amino acid pathways at plateau period were upregulated, while host material catabolic and bacterial motility-related genes were downregulated, indicating that ΦAFP1 might hijack the energy of the host for the synthesis of phage proteins. ΦAFP1 exerted the step-by-step control on host genes through the appropriate level of the utilizing host resources, affirming a new non-standard regulatory strategy of viral temperately control over the host transcriptional profile. Our study provides novel information for a better understanding of filamentous phage characteristics and phage-host interactions.
更新日期：2022-06-27
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