Glucosinolates and camalexin are secondary metabolites that, as phytoanticipins and phytoalexins, play a crucial role in plant defence. The present work proposes an improved analytical method for routine analysis and quantification of glucosinolates and camalexin in brassicaceous small-sized samples by using the very specific desulfation process of glucosinolates analysis and the specificity of fluorescence detection for camalexin analysis. The approach is based on a simultaneous ultrasound-assisted extraction followed by a purification on an anion-exchange column. Final analyses are conducted by HPLC-UV-MS for desulfo-glucosinolates and HPLC coupled to a fluorescence detector (HPLC-FLD) for camalexin. The method is linear for glucosinolates (50–3500 µM) and camalexin (0.025–5 µg.mL⁻¹) with an LOD/LOQ of 3.8/12.6 µM and 0.014/0.046 µg.mL⁻¹ respectively. The method demonstrated adequate precision, accuracy and trueness on certified reference rapeseed. A practical application of our approach was conducted on different Brassicaceae genera (Barbarea vulgaris, Brassica nigra, Capsella bursa-pastoris, Cardamine hirsuta, Coincya monensis, Sinapis arvensis, and Sisymbrium officinale) and Arabidopsis thaliana genotypes (Columbia and Wassilewskija). Futhermore, different plant organs (seeds and leaves) were analysed, previously inoculated or not with the pathogenic fungus Alternaria brassicicola.

硫代葡萄糖苷和camalexin 是次生代谢产物，与植物抗毒素和植物抗毒素一样，在植物防御中起着至关重要的作用。本工作提出了一种改进的分析方法，利用硫代葡萄糖苷分析的非常特殊的脱硫过程和荧光检测的特异性，对芸苔属小尺寸样品中的硫代葡萄糖苷和camalexin 进行常规分析和定量。该方法基于同时进行的超声辅助萃取，然后在阴离子交换柱上进行纯化。最终分析通过 HPLC-UV-MS 进行脱硫-硫代葡萄糖苷和 HPLC 耦合到荧光检测器 (HPLC-FLD) 的camalexin。该方法对硫代葡萄糖苷 (50–3500 µM) 和 camalexin (0.025–5 µg.mL ^{-1 ) 呈线性}) 的 LOD/LOQ 分别为 3.8/12.6 µM 和 0.014/0.046 µg.mL ^-1。该方法在认证的参考油菜籽上表现出足够的精密度、准确度和真实性。我们的方法在不同的十字花科属（Barbarea vulgaris、Brassica nigra、Capsella bursa-pastoris、Cardamine hirsuta、Coincya monensis、Sinapis arvensis和Sisymbrium officinale）和拟南芥基因型（哥伦比亚和 Wassilewskija）上进行了实际应用。此外，分析了不同的植物器官（种子和叶子），之前接种过或未接种过病原真菌Alternaria brasicicola。