3D spheroids, which have the potential to bridge the gap between 2D cell culture and native tissue, are used as tissue models in many applications, particularly in cancer, stem cell, and pharmaceutical research. A considerable amount of effort has focused on the development of more relevant physiological models. However, spheroids still have limitations in that they cannot replicate the components and structure of the ECM in the natural environment. In this study, we proposed new concept of scaffold-based techniques for the generation of spheroids. Spheroids were successfully generated by single cell or small number of aggregated cells between HA particles. The size of each spheroid was uniform, a necrotic core didn't form, and the system showed high viability. The expression levels of the proteins and genes required to maintain cell-specific functions increased. Thus, our system provides more physiologically relevant models and could be applied to regenerative medicine or drug screening.

3D 球体有可能弥合 2D 细胞培养和天然组织之间的差距，在许多应用中用作组织模型，特别是在癌症、干细胞和药物研究中。相当多的努力集中在开发更相关的生理模型上。然而，球体仍然有局限性，因为它们无法在自然环境中复制 ECM 的组件和结构。在这项研究中，我们提出了基于支架技术生成球体的新概念。球体是由 HA 颗粒之间的单个细胞或少量聚集细胞成功生成的。每个球体大小均一，没有形成坏死核，系统显示出较高的生存能力。维持细胞特异性功能所需的蛋白质和基因的表达水平增加。因此，我们的系统提供了更多生理相关的模型，可应用于再生医学或药物筛选。