Journal of Advanced Research ( IF 11.4 ) Pub Date : 2022-06-25 , DOI: 10.1016/j.jare.2022.06.012 Masoud Hamidi 1 , Oseweuba Valentine Okoro 2 , Giuseppe Ianiri 3 , Hafez Jafari 2 , Khodabakhsh Rashidi 4 , Saeed Ghasemi 5 , Raffaello Castoria 3 , Davide Palmieri 3 , Cédric Delattre 6 , Guillaume Pierre 7 , Mahta Mirzaei 2 , Lei Nie 8 , Hadi Samadian 9 , Amin Shavandi 2
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Introduction
Exopolysaccharides (EPSs) are high-value functional biomaterials mainly produced by bacteria and fungi, with nutraceutical, therapeutic and industrial potentials.
Objectives
This study sought to characterize and assess the biological properties of the EPS produced by the yeast Papiliotrema terrestris PT22AV.
Methods
After extracting the yeast's DNA and its molecular identification, the EPS from P. terrestris PT22AV strain was extracted and its physicochemical properties (structural, morphological, monosaccharide composition and molecular weight) were characterized. The EPS's in vitro biological activities and in vivo wound healing potential were also evaluated.
Results
The obtained EPS was water-soluble and revealed an average molecular weight (Mw) of 202 kDa. Mannose and glucose with 97% and 3% molar percentages, respectively, constituted the EPS. In vitro antibacterial activity analysis of the extracted EPS exhibited antibacterial activity (>80%) against Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis at a concentration of 2 mg/mL. The EPS showed cytocompatibility against the human fibroblast and macrophage cell lines and the animal studies showed a dose-dependent wound healing capacity of the EPS with higher wound closure at 10 mg/mL compared to negative and positive control after 14 days.
Conclusion
The EPS from P. terrestris PT22AV could serve as a promising source of biocompatible macromolecules with potential for skin wound healing.
中文翻译：
来自酵母 Papiliotrema terrestris PT22AV 的胞外多糖用于皮肤伤口愈合
介绍
胞外多糖（EPSs）是一种高价值的功能性生物材料，主要由细菌和真菌产生，具有营养、治疗和工业潜力。
目标
本研究旨在表征和评估由酵母菌土生乳突PT22AV产生的 EPS 的生物学特性。
方法
在提取酵母 DNA 并对其进行分子鉴定后，提取了来自P. terrestris PT22AV 菌株的 EPS，并对其理化性质（结构、形态、单糖组成和分子量）进行了表征。还评估了EPS 的体外生物活性和体内伤口愈合潜力。
结果
获得的 EPS 是水溶性的，平均分子量 ( Mw )为 202 kDa。分别具有 97% 和 3% 摩尔百分比的甘露糖和葡萄糖构成了 EPS。提取的 EPS 的体外抗菌活性分析显示，浓度为 2 mg/mL 时，对大肠杆菌、金黄色葡萄球菌和表皮葡萄球菌具有抗菌活性 (>80%)。EPS 显示出与人成纤维细胞和巨噬细胞系的细胞相容性，动物研究表明 EPS 具有剂量依赖性伤口愈合能力，与阴性和阳性对照相比，14 天后 10 mg/mL 的伤口闭合率更高。
结论
来自P. terrestris PT22AV的 EPS可以作为具有皮肤伤口愈合潜力的生物相容性大分子的有前途的来源。
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