The accumulation of microplastics in marine organisms is an emerging concern. Due to trophic transfer, the safety of seafood is under investigation in view of the potential negative effects of microplastics on human health. In this study, market samples of Manila clams (Ruditapes philippinarum) from South Korea were segregated into two groups of considerably different size (p < 0.05), namely small clams with shell length of 40.69 ± 3.97 mm, and large clams of shell length 51.19 ± 2.86 mm. Comparative profiling of the number, size, shape, and polymer type of microplastics were performed using μFTIR imaging and Nile red staining. Overall, μFTIR detected only 1559 microplastics while 1996 microplastics were counted based on staining from 61 Manila clams (30 small and 31 large), leading to an overestimation of 18 to 75 %. Comparable microplastics concentration, based on μFTIR, were observed at 2.70 ± 1.66 MP/g or 15.64 ± 9.25 MP/individual for the small samples, and 3.65 ± 1.59 MP/g or 41.63 ± 16.90 MP/individual for the large ones (p > 0.05). Particle diameters of 20–100 μm was the most dominant, accounting for 44.6 % and 46.5 % of all microplastics from the small and large groups, respectively. Particles, with a circularity (resemblance to a circle) value between 0.6 and 1.0, were the most prevalent, followed by fragments and fibers. At least 50 % of microplastics from the small and large samples were polystyrene, making it the most abundant polymer type. Despite the substantial difference in the size of the animals, only a weak to moderate correlation was observed between microplastics content and the physical attributes of the clams such as shell length and weight, (soft) tissue weight, and total weight (Spearman's coefficient < 0.5). The estimated intake of microplastics by the Korean population was 1232 MP/person/year via small clams, 1663 MP/person/year via large clams, and 1489 MP/person/year via clams independent of size.

海洋生物中微塑料的积累是一个新兴的问题。由于营养转移，鉴于微塑料对人类健康的潜在负面影响，海鲜的安全性正在接受调查。在这项研究中，来自韩国的马尼拉蛤蜊 ( Ruditapes philippinarum ) 的市场样本被分成两组大小差异很大 ( p < 0.05)，即壳长为 40.69 ± 3.97 mm 的小蛤蜊和壳长 51.19 ± 2.86 mm 的大蛤蜊。使用 μFTIR 成像和尼罗红染色对微塑料的数量、大小、形状和聚合物类型进行比较分析。总体而言，μFTIR 仅检测到 1559 个微塑料，而 1996 个微塑料是根据 61 个马尼拉蛤（30 个小和 31 个大）的染色计算得出的，导致高估了 18% 到 75%。基于 μFTIR，可比较的微塑料浓度观察到小样品为 2.70 ± 1.66 MP/g 或 15.64 ± 9.25 MP/个，大样品为 3.65 ± 1.59 MP/g 或 41.63 ± 16.90 MP/个 ( p > 0.05)。20-100 μm 的粒径占主导地位，分别占小组和大组所有微塑料的 44.6% 和 46.5%。圆形度（类似于圆形）值在 0.6 和 1.0 之间的颗粒最为普遍，其次是碎片和纤维。来自小型和大型样品的至少 50% 的微塑料是聚苯乙烯，使其成为最丰富的聚合物类型。尽管动物的体型存在显着差异，但在微塑料含量与蛤蜊的物理属性（如壳长和重量、（软）组织重量和总重量）之间仅观察到弱到中等的相关性（Spearman 系数 < 0.5 ）。估计韩国人口通过小蛤蜊摄入的微塑料为 1232 MP/人/年，