Band-Edge Effect-Induced Electrochemiluminescence Signal Amplification Based on Inverse Opal Photonic Crystals for Ultrasensitive Detection of Carcinoembryonic Antigen,Analytical Chemistry

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Band-Edge Effect-Induced Electrochemiluminescence Signal Amplification Based on Inverse Opal Photonic Crystals for Ultrasensitive Detection of Carcinoembryonic Antigen
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-06-24 , DOI: 10.1021/acs.analchem.2c01986
Xin Zhang _{1,

2} , Zhong-Jie Wang ₁ , Xu Wang ₁ , Ya-Heng Zhang ₁ , Jian Qu ₁ , Shou-Nian Ding ₂

Affiliation

Photonic crystals (PCs) have emerged as a promising electrochemiluminescence (ECL) matrix in the domain of immunoassay. Making maximum use of light manipulation properties of PCs is highly desired for improving the sensitivity. In this work, we proposed a band-edge effect-induced ECL enhancement strategy based on silica inverse opal PCs (SIOPCs). By fine-tuning the lattice constant and carefully calibrating the stopband position, we found that the band edge of the stopband exerted significant influences on the ECL intensity and spectral distribution. The high density of states at the blue edge of the photonic band gap increased the radiative transition probability of ECL emitters and enhanced the photon extraction during propagation, giving rise to ∼20-fold ECL signal amplification accompanied by a redistributed ECL spectrum for the Ru(bpy)₃²⁺-TPrA system. In combination with the intrinsic structural superiority, like large specific surface area and interconnected macropores, the developed SIOPC electrode was successfully applied in constructing a sandwich-type immunosensor. The fabricated immunosensor displayed a very low detection limit of 0.032 pg/mL and a wide linear range of 0.1 pg/mL–150 ng/mL for a carcinoembryonic antigen assay, showing its potential application in disease diagnosis.

中文翻译：

基于反蛋白石光子晶体的带边效应诱导电化学发光信号放大用于癌胚抗原的超灵敏检测

光子晶体 (PC) 已成为免疫测定领域中一种很有前途的电化学发光 (ECL) 基质。为了提高灵敏度，非常需要最大限度地利用 PC 的光操纵特性。在这项工作中，我们提出了一种基于二氧化硅反蛋白石 PC (SIOPC) 的带边效应诱导的 ECL 增强策略。通过微调晶格常数并仔细校准阻带位置，我们发现阻带的带边缘对 ECL 强度和光谱分布产生显着影响。光子带隙蓝色边缘的高密度状态增加了 ECL 发射器的辐射跃迁概率并增强了传播过程中的光子提取，导致 ECL 信号放大约 20 倍，伴随着重新分布的 ECL 光谱。 bpy)₃²⁺ -TPrA 系统。结合其固有的结构优势，如大的比表面积和相互连接的大孔，所开发的SIOPC电极成功地应用于构建三明治型免疫传感器。制造的免疫传感器在癌胚抗原测定中显示出非常低的 0.032 pg/mL 检测限和 0.1 pg/mL–150 ng/mL 的宽线性范围，显示其在疾病诊断中的潜在应用。

更新日期：2022-06-24

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