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Fentanyl Assay Derived from Intermolecular Interaction-Enabled Small Molecule Recognition (iMSR) with Differential Impedance Analysis for Point-of-Care Testing
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-06-23 , DOI: 10.1021/acs.analchem.2c00017 Zhe Wang 1 , Amit Nautiyal 2 , Christopher Alexopoulos 1 , Rania Aqrawi 1 , Xiaozhou Huang 3 , Ashraf Ali 4 , Katherine E Lawson 4 , Kevin Riley 2 , Andrew J Adamczyk 4 , Pei Dong 3 , Xinyu Zhang 4
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-06-23 , DOI: 10.1021/acs.analchem.2c00017 Zhe Wang 1 , Amit Nautiyal 2 , Christopher Alexopoulos 1 , Rania Aqrawi 1 , Xiaozhou Huang 3 , Ashraf Ali 4 , Katherine E Lawson 4 , Kevin Riley 2 , Andrew J Adamczyk 4 , Pei Dong 3 , Xinyu Zhang 4
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Rapid and effective differentiation and quantification of a small molecule drug, such as fentanyl, in bodily fluids are major challenges for diagnosis and personal medication. However, the current toxicology methods used to measure drug concentration and metabolites require laboratory-based testing, which is not an efficient or cost-effective way to treat patients in a timely manner. Here, we show an assay for monitoring fentanyl levels by combining the intermolecular interaction-enabled small molecule recognition (iMSR) with differential impedance analysis of conjugated polymers. The differential interactions with the designed anchor interface were transduced through the perturbance of the electric status of the flexible conducting polymer. This assay showed excellent fentanyl selectivity against common interferences, as well as in variable body fluids through either testing strips or skin patches. Directly using the patient blood, the sensor provided 1%–5% of the average deviation compared to the “gold” standard method LC-MS results in the medically relevant fentanyl range of 20–90 nM. The superior sensing properties, in conjunction with mechanical flexibility and compatibility, enabled point-of-care detection and provided a promising avenue for applications beyond the scope of biomarker detection.
中文翻译：
芬太尼检测源自支持分子间相互作用的小分子识别 (iMSR) 以及用于床旁检测的差分阻抗分析
对体液中的小分子药物（如芬太尼）进行快速有效的区分和量化是诊断和个人用药的主要挑战。然而，目前用于测量药物浓度和代谢物的毒理学方法需要基于实验室的测试，这不是及时治疗患者的有效或经济有效的方法。在这里，我们展示了一种通过将分子间相互作用小分子识别 (iMSR) 与共轭聚合物的差分阻抗分析相结合来监测芬太尼水平的方法。通过柔性导电聚合物的电状态的扰动来转换与设计的锚界面的微分相互作用。该测定对常见的干扰物表现出优异的芬太尼选择性，以及通过测试条或皮肤贴片在可变的体液中。与“金”标准方法 LC-MS 相比，传感器直接使用患者血液提供 1%–5% 的平均偏差，结果医学相关的芬太尼范围为 20–90 nM。卓越的传感特性与机械灵活性和兼容性相结合，实现了即时检测，并为生物标志物检测范围之外的应用提供了有希望的途径。
更新日期：2022-06-23
中文翻译：
芬太尼检测源自支持分子间相互作用的小分子识别 (iMSR) 以及用于床旁检测的差分阻抗分析
对体液中的小分子药物（如芬太尼）进行快速有效的区分和量化是诊断和个人用药的主要挑战。然而，目前用于测量药物浓度和代谢物的毒理学方法需要基于实验室的测试，这不是及时治疗患者的有效或经济有效的方法。在这里，我们展示了一种通过将分子间相互作用小分子识别 (iMSR) 与共轭聚合物的差分阻抗分析相结合来监测芬太尼水平的方法。通过柔性导电聚合物的电状态的扰动来转换与设计的锚界面的微分相互作用。该测定对常见的干扰物表现出优异的芬太尼选择性，以及通过测试条或皮肤贴片在可变的体液中。与“金”标准方法 LC-MS 相比，传感器直接使用患者血液提供 1%–5% 的平均偏差，结果医学相关的芬太尼范围为 20–90 nM。卓越的传感特性与机械灵活性和兼容性相结合，实现了即时检测，并为生物标志物检测范围之外的应用提供了有希望的途径。
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