Integrated Approach to Extract and Purify Proteins from Honey by Ionic Liquid-Based Three-Phase Partitioning,ACS Sustainable Chemistry & Engineering

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Integrated Approach to Extract and Purify Proteins from Honey by Ionic Liquid-Based Three-Phase Partitioning
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2022-06-24 , DOI: 10.1021/acssuschemeng.2c01782
Matheus M Pereira ₁ , Sónia N Pedro ₁ , Maria V Quental ₁ , Aminou Mohamadou ₂ , João A P Coutinho ₁ , Mara G Freire ₁

Affiliation

The purification of value-added compounds by three-phase partitioning (TPP) is a promising alternative to conventional processes since the target compound can be easily recovered from the liquid–liquid interphase. Although this technique has been successfully applied to the recovery of proteins, the minimization of the use of salts and solvents must be pursued to improve the overall process sustainability. Accordingly, we have here investigated the use of biobased glycine-betaine ionic liquids (IL) directly with honey, a carbohydrate-rich matrix, as phase-forming components of TPP systems. These ILTPP systems were applied in the purification of major royal jelly proteins (MRJPs) from honey. The results obtained show that MRJPs mostly precipitate in the ILTPP interphase, with a recovery yield ranging between 82.8% and 97.3%. In particular, MRJP1 can be obtained with a purity level up to 90.1%. Furthermore, these systems allow the simultaneous separation of antioxidants and carbohydrates to different liquid phases. The proposed approach allows the separation of proteins, antioxidants, and carbohydrates from honey in a single step, while using only ILs and a real carbohydrate-rich matrix, thus being sustainable TPP processes.

中文翻译：

基于离子液体的三相分配从蜂蜜中提取和纯化蛋白质的综合方法

通过三相分配 (TPP) 纯化增值化合物是传统工艺的一种有前途的替代方法，因为目标化合物可以很容易地从液-液界面中回收。尽管该技术已成功应用于蛋白质的回收，但必须尽量减少盐和溶剂的使用，以提高整个过程的可持续性。因此，我们在这里研究了生物基甘氨酸-甜菜碱离子液体 (IL) 与蜂蜜（一种富含碳水化合物的基质）直接作为 TPP 系统的相形成组分的用途。这些 ILTPP 系统应用于从蜂蜜中纯化主要蜂王浆蛋白 (MRJP)。获得的结果表明，MRJPs 主要沉淀在 ILTPP 界面，回收率在 82.8% 和 97.3% 之间。特别是，可以获得纯度高达 90.1% 的 MRJP1。此外，这些系统允许同时将抗氧化剂和碳水化合物分离到不同的液相中。所提出的方法允许在一个步骤中从蜂蜜中分离蛋白质、抗氧化剂和碳水化合物，同时仅使用离子液体和真正富含碳水化合物的基质，因此是可持续的 TPP 过程。

更新日期：2022-06-24

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