Effects of hydrocarbon chain on the vesicle size distribution, kinetics of average size, bending modulus, and elastic modulus of lipid membranes,The European Physical Journal E

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Effects of hydrocarbon chain on the vesicle size distribution, kinetics of average size, bending modulus, and elastic modulus of lipid membranes
The European Physical Journal E ( IF 1.8 ) Pub Date : 2022-06-24 , DOI: 10.1140/epje/s10189-022-00209-y
Md Imran Hossain ₁ , Mohammad Abu Sayem Karal ₂ , Md Kamruzzaman ₁ , Ishtiaque M Syed ₁ , Sharif Hasan ₂ , Md Kabir Ahamed ₂

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The effects of the hydrocarbon chain of lipids on the size distribution of giant unilamellar vesicles (GUVs), kinetics of average size, bending modulus, and elastic modulus of membranes have been investigated. 1,2-dioleoyl-sn-glycero-3-phosphocholine (18:1 (Δ9-Cis) PC (DOPC)), 1,2-dipalmitoleoyl-sn-glycero-3-phosphocholine (16:1 (Δ9-Cis) PC), and 1,2-ditridecanoyl-sn-glycerol-3-phosphocholine (13:0 PC (DTPC)) lipids were considered. The number of hydrocarbons in a chain of the corresponding lipid was 18, 16, and 13. GUVs were prepared using the natural swelling method under incubation times of 20, 40, 60, 90, 120, and 180 min. The size distribution of vesicles was fitted using the lognormal distribution. The average sizes of DOPC, 16:1 (Δ9-Cis) PC, and DTPC-GUVs increased with the incubation time until 120 min, and then remained steady at 16.7 ± 0.2, 15.2 ± 0.4 and 12.0 ± 0.3 µm for the corresponding lipids. The average size at equilibrium state increased with the number of hydrocarbons. The incubation time-dependent average size was fitted with an exponential growth equation, and then the kinetic constants of 0.028 ± 0.004, 0.036 ± 0.007, and 0.083 ± 0.009 min⁻¹ for DOPC, 16:1 (Δ9-Cis) PC, and DTPC-GUVs, respectively, were obtained. The equilibrium size distribution was fitted by the theoretical equation, and the bending modulus for DOPC, 16:1 (Δ9-Cis) PC, and DTPC membranes were 19.5 ± 0.2, 18.5 ± 0.1 and 14.3 ± 0.1 k_BT, respectively. The bending modulus increased with the number of hydrocarbons. The elastic modulus of these membranes was 261 mN/m with a 4% fluctuation. The correlation between the average size and the square root of the bending modulus was supported by theoretical analysis.

Graphical abstract

中文翻译：

烃链对脂质膜囊泡尺寸分布、平均尺寸动力学、弯曲模量和弹性模量的影响

研究了脂质烃链对巨型单层囊泡 (GUV) 的尺寸分布、平均尺寸动力学、弯曲模量和膜弹性模量的影响。1,2-dioleoyl - sn -glycero-3-phosphocholine (18:1 (Δ9-Cis) PC (DOPC)), 1,2-dipalmitoleoyl- sn- glycero -3 -phosphocholine (16:1 (Δ9-Cis)) PC) 和 1,2-二十三烷酰基-sn考虑了-glycerol-3-phosphocholine (13:0 PC (DTPC)) 脂质。相应脂质链中碳氢化合物的数量为 18、16 和 13。使用自然溶胀法在 20、40、60、90、120 和 180 分钟的孵育时间下制备 GUV。使用对数正态分布拟合囊泡的大小分布。DOPC、16:1 (Δ9-Cis) PC 和 DTPC-GUV 的平均大小随着孵育时间的增加而增加，直到 120 分钟，然后对于相应的脂质保持稳定在 16.7 ± 0.2、15.2 ± 0.4 和 12.0 ± 0.3 µm . 平衡状态下的平均尺寸随着烃类数量的增加而增加。孵育时间相关的平均大小符合指数增长方程，然后是 0.028 ± 0.004、0.036 ± 0.007 和 0.083 ± 0.009 min ^{-1的动力学常数}对于 DOPC，分别获得了 16:1 (Δ9-Cis) PC 和 DTPC-GUV。平衡尺寸分布由理论方程拟合，DOPC、16:1 (Δ9-Cis) PC 和 DTPC 膜的弯曲模量分别为 19.5 ± 0.2、18.5 ± 0.1 和 14.3 ± 0.1 k _BT。弯曲模量随着碳氢化合物数量的增加而增加。这些膜的弹性模量为 261 mN/m，波动率为 4%。理论分析支持平均尺寸与弯曲模量平方根之间的相关性。

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更新日期：2022-06-24

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